Epsigam fusion protein

A method for manufacturing a fusion protein, comprising

• synthesizing a nucleotide sequence comprising of a first restriction site at a first end of the nucleotide sequence and a second restriction site at a second end of the nucleotide sequence;
• inserting the nucleotide sequence into a cloning vector;
• excising the nucleotide sequence from the cloning vector using a first restriction enzyme corresponding to said first restriction site and a second restriction enzyme corresponding to the second restriction site;
• inserting the nucleotide sequence into an expression vector, wherein said expression vector links the expression of an exogenous protein to the glutamine synthetase gene;
• transfecting cells with the expression vector using electroporation, wherein the cells are deficient in glutamine synthetase;
• incubating the transfected cells;
• and
• collecting a secreted protein from the cells comprising said fusion protein.