US 2015/0307574 A1Published application

Modified galectin-9 protein

AssigneeNational University Corporation Kagawa University

InventorsNozomu NISHI, Aiko ITOH

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Abstract

The present invention provides galectin-9 variant having substantially the same bioactivity as in wild-type galectin-9 and being superior in protease stability, solubility, and yield. The galectin-9 variant according to the present invention is a protein including the following NCRD and the following CCRD composed of an N-terminal region and a C-terminal region. The C terminus of the NCRD and the N terminus of the CCRD are directly or indirectly bound to each other. The NCRD is (N1) a peptide composed of an amino acid sequence represented by SEQ ID NO: 1. The N-terminal region of the CCRD is (C-N1) a peptide composed of an amino acid sequence obtained by deletion of 1 to 17 amino acids in an amino acid sequence represented by SEQ ID NO: 3. The C-terminal region is (C-C1) a peptide composed of an amino acid sequence represented by SEQ ID NO: 5.

Claims27

Dependent claims

A protein or a salt thereof, comprising:

NCRD;
and
CCRD, a C terminus of the NCRD and an N terminus of the CCRD being directly bound to each other, wherein
the NCRD is
(N1) a peptide composed of an amino acid sequence represented by SEQ ID NO:
1 or
(N2) a peptide which is composed of an amino acid sequence obtained by deletion, substitution and/or insertion of one or more amino acids in the amino acid sequence represented by SEQ ID NO:
1 and has a carbohydrate binding ability, and
the CCRD is
a peptide composed of an N-terminal region and a C-terminal region and has a carbohydrate binding ability, wherein
the N-terminal region is
(C-N1) a peptide composed of an amino acid sequence obtained by deletion of 1 to 17 amino acids in an amino acid sequence represented by SEQ ID NO:
3, and
the C-terminal region is
(C-C1) a peptide composed of an amino acid sequence represented by SEQ ID NO:
5 or
(C-C2) a peptide composed of an amino acid sequence obtained by deletion, substitution and/or insertion of one or more amino acids in the amino acid sequence represented by SEQ ID NO:
5.

15 more elements

Dependencies are inferred from the claim text.