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US 2015/0307566 A1Published application
Vaccine and methods for detecting and preventing filariasis
AssigneeThe Board of Trustees of the University of Illinois
InventorRamaswamy Kalyanasundaram
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Abstract
The present invention is a multivalent vaccine for immunizing an animal against filariasis. In some embodiments, the antigens of the multivalent vaccine are protein-based, DNA-based, or a combination thereof. This invention also provides a method and kit for detecting a filarial nematode and determining vaccine efficacy.
Claims20
A fusion protein comprising
- (a) Brugia malayi Abundant Larval Transcript;
- and
- (b) Brugia malayi Small heat shock protein 12.6, Brugia malayi Tetraspanin, Brugia malayi Thioredoxin Peroxidase 2, or a combination thereof.
A vaccine comprising
- (a) an Abundant Larval Transcript protein comprising SEQ ID NO:78 or SEQ ID NO:79;
- and
- (b) a Small heat shock protein 12.6 comprising SEQ ID NO:80 or SEQ ID NO:81;
- a Tetraspanin protein comprising SEQ ID NO:82;
- a Thioredoxin Peroxidase 2 protein comprising SEQ ID NO:83 or SEQ ID NO:84, or a combination thereof.
2 more elements
A method for detecting the presence of a filarial nematode comprising
- (a) contacting a biological sample, in vitro, with one or more binding agents against filarial nematode proteins selected from the group of ALT2, TSP, VAL-1, TPX2 and HSP or fragments thereof;
- and
- (b) detecting binding between the binding agents and the filarial nematode proteins, wherein the presence of binding between the binding agents and the filarial nematode proteins indicates the presence of a filarial nematode.
A kit comprising
- (a) one or more binding agents against filarial nematode proteins selected from the group of ALT2, TSP, VAL-1, TPX2 and HSP or fragments thereof;
- (b) a device with a solid surface.
A method for determining the presence of antibodies to filarial nematode proteins comprising
- (a) contacting one or more filarial nematode proteins selected from the group of ALT2, TSP, VAL-1, TPX2 and HSP, or fragments thereof, with a biological sample suspected of containing antibodies to one or more of the filarial nematode proteins;
- and
- (b) detecting binding between the one or more filarial nematode proteins and antibodies in the biological sample, wherein the presence of antibodies to the one or more filarial nematode proteins is indicative of efficacy of a vaccine to the filarial nematode, prior exposure to filarial proteins, or an existing infection with a filarial nematode.
A kit comprising
- (a) one or more filarial nematode proteins selected from the group of ALT2, TSP, VAL-1, TPX2 and HSP, or fragments thereof;
- (b) a device with one or more solid surfaces.
Dependencies are inferred from the claim text.