Strategy to engineer phytochromes with improved action in crop fields
AssigneeWisconsin Alumni Research Foundation
InventorsErnest Sethe Burgie, Adam Nicholas Bussell, Richard David Vierstra, Joseph Walker
Life of this patent
Estimating the term…
Abstract
Disclosed herein are to isolated polynucleotide sequences encoding modified phytochrome polypeptides wherein the modified phytochrome polypeptide has at least one of an altered thermal reversion rate, an altered photoconversion rate, an altered absorption spectrum, or an altered signal output compared to the unmodified phytochrome polypeptide. Also disclosed are transgenic plants comprising said modified phytochrome polypeptides and methods of producing said transgenic plants.
Claims22
An isolated polynucleotide encoding a modified phytochrome polypeptide comprising an amino acid sequence that is at least 80% identical to an unmodified phytochrome polypeptide and having at least one amino acid substitution at a position corresponding to position 104, 108, 284, 358, 401, 403, 563, 565, 584, or a combination thereof, of SEQ ID NO:1, the unmodified phytochrome polypeptide having an amino acid sequence selected from SEQ ID NOs:
- 1-26 or 67-92.
-25. (canceled)
-35. (canceled)
The plant of claim 35, wherein the plant is corn, soybean or rice.
The plant of claim 35, wherein the plant is an ornamental plant.
A method of producing a transgenic plant, the method comprising:
- (a) introducing into a plant cell an isolated polynucleotide encoding a modified phytochrome polypeptide comprising an amino acid sequence that is at least 80% identical to an unmodified phytochrome polypeptide and having at least one amino acid substitution at a position corresponding to position 104, 108, 284, 358, 401, 403, 563, 565, 584, or a combination thereof, of SEQ ID NO:1, the unmodified phytochrome polypeptide having an amino acid sequence selected from SEQ ID NOs:
- 1-26 or 67-92;
- and
- (b) regenerating the transformed cell to produce a transgenic plant.
-42. (canceled)
The method of claim 42, wherein the transgenic plant is a corn, soybean or rice plant.
The method of claim 42, wherein the transgenic plant is an ornamental plant.
An isolated polypeptide comprising an amino acid sequence that is at least 80% identical to an unmodified phytochrome polypeptide and having at least one amino acid substitution at a position corresponding to position 104, 108, 284, 358, 401, 403, 563, 565, 584, or a combination thereof, of SEQ ID NO:1, the unmodified phytochrome polypeptide having an amino acid sequence selected from SEQ ID NOs:
- 1-26 or 67-92.
Dependencies are inferred from the claim text.