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AssigneePronutria Biosciences, Inc.
InventorsSubhayu Basu, Katherine G. Gora, Ying-Ja Chen, David M. Young, Nathaniel W. Silver, Michael J. Hamill, David A. Berry
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Nutritive proteins are provided herein. Also provided are various other embodiments including nucleic acids encoding the proteins, recombinant microorganisms that make the proteins, vectors for expressing the proteins, methods of making the proteins using recombinant microorganisms, compositions that comprise the proteins, and methods of using the proteins. Nutritive proteins include engineered proteins, wherein the engineered proteins comprise a sequence of at least 20 amino acids that comprise an altered amino acid sequence compared to the amino acid sequence of a reference secreted protein and a ratio of essential amino acids to total amino acids present in the engineered protein higher than the ratio of essential amino acids to total amino acids present in the reference secreted protein. In some embodiments, the engineered protein comprises at least one essential amino acid residue substitution of a non-essential amino acid residue in the reference secreted protein.
A formulation comprising an isolated nutritive polypeptide, wherein the nutritive polypeptide comprises a ratio of one or more essential amino acids to total amino acids that is higher than the ratio of one or more essential amino acids to total amino acids in a reference secreted protein at least 50 amino acids in length, wherein the nutritive polypeptide is present in the formulation in a nutritional amount, and wherein the formulation is substantially free of non-comestible products.
A recombinant unicellular organism comprising a recombinant nucleic acid sequence encoding an isolated nutritive polypeptide, wherein the nutritive polypeptide comprises a ratio of one or more essential amino acids to total amino acids that is higher than the ratio of one or more essential amino acids to total amino acids in a reference secreted protein at least 50 amino acids in length.
A method of formulating a nutritive product, comprising the steps of providing a composition comprising an effective amount of an isolated nutritive polypeptide, wherein the nutritive polypeptide comprises a ratio of one or more essential amino acids to total amino acids that is higher than the ratio of one or more essential amino acids to total amino acids in a reference secreted protein at least 50 amino acids in length, wherein the nutritive polypeptide is present in the composition at a concentration of at least 1 mg of nutritive polypeptide per gram of the composition, and combining the composition with at least one food component, thereby formulating the nutritive product.
A method of selecting a nutritive composition for administration to a human subject who can benefit from same, the method comprising:
A method of selecting a nutritive composition for administration to a human subject who can benefit from same, the method comprising:
A method of treating a disease, disorder or condition characterized or exacerbated by protein malnourishment in a human subject in need thereof, comprising the step of administering to the human subject a nutritive formulation in an amount sufficient to treat such disease, disorder or condition, wherein the nutritive formulation comprises a nutritive polypeptide and an agriculturally-derived food product, wherein the nutritive polypeptide comprises a ratio of one or more essential amino acids to total amino acids that is higher than the ratio of one or more essential amino acids to total amino acids in a reference secreted protein at least 50 amino acids in length.
A method of improving the nutritional status of a human subject, comprising administering to the subject an effective amount of a nutritive formulation comprising an agriculturally-derived food product and an isolated nutritive polypeptide, wherein the nutritive polypeptide comprises a ratio of one or more essential amino acids to total amino acids that is higher than the ratio of one or more essential amino acids to total amino acids in a reference secreted protein at least 50 amino acids in length.
An engineered protein comprising:
An engineered protein comprising at least one essential amino acid residue substitution of a non-essential amino acid residue in a reference secreted protein at a position with a position entropy of at least 1.5.
An isolated engineered protein comprising a sequence of at least 20 amino acids that comprise an altered amino acid sequence compared to the amino acid sequence of a reference secreted protein and a ratio of essential amino acids to total amino acids present in the engineered protein higher than the ratio of essential amino acids to total amino acids present in the reference secreted protein.
A method of making an engineered protein, comprising:
A library comprising a plurality of recombinant nucleic acid sequences encoding nutritive polypeptide variants, wherein each nutritive polypeptide variant comprises a ratio of one or more essential amino acids to total amino acids that is higher than the ratio of one or more essential amino acids to total amino acids in a reference secreted protein at least 50 amino acids in length.
Dependencies are inferred from the claim text.