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US 2015/0307547 A1Published application
Egg White Processing
AssigneeSynageva BioPharma Corp.
InventorsLiang Chen, Markley C. Leavitt, Michael Titus
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Abstract
This disclosure relates to inexpensive and efficient methods of preparing egg white (e.g., obtained from eggs laid by transgenic chickens) for bulk chromatographic isolation of proteins (e.g., recombinant proteins) from the egg white, as well as methods of filtering acidified egg white and methods of isolating proteins from the egg white.
Claims50
A method of preparing egg white for bulk chromatographic processing, the method comprising the steps of:
- adding an acidic buffer comprising an acidic agent to a pool of egg white, the acidic buffer being from about 0.5 wt % to about 5 wt % per kilogram of the egg white;
- and
- mixing the acidic buffer and the egg white to form a mixed egg white having a pH from about 5 to about 6.5.
A method of isolating a recombinant protein from egg white, comprising the steps of:
- providing a pool of egg white comprising a recombinant protein, the pool having a volume of at least about 10 liters;
- adjusting the pH of the egg white to from about 5 to about 6.5, wherein the conductivity of the pH-adjusted egg white is from about 8 mS/cm to about 20 mS/cm;
- filtering the egg white to form a solution;
- and
- isolating the recombinant protein in the egg white by column chromatography.
2 more elements
A method of filtering acidified egg white, comprising the steps of:
- passing a pre-treatment buffer having a conductivity between about 8 mS/cm and about 20 mS/cm through a filter;
- and
- passing egg white having a pH from about 5 to about 6.5 through the filter to obtain a filtered egg white.
Dependencies are inferred from the claim text.