Thermo-responsive lectin-elp fusion binding ligands for glycoprotein purification by affinity precipitation
AssigneeGeorgia Tech Research Corporation
InventorsRachel Ruizhen Chen, Lindsay Grace Arnold
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Abstract
The invention provides novel affinity fusion ligands, and method of use thereof, for glycoprotein purification by affinity precipitation. The affinity fusion ligand of the invention comprises a bacterial lectin, e.g., from Ralstonia solanacearum (RSL) that binds to fucose, and/or from the Vibrio cholera neuraminidase (VCNA) that binds to saialic acid, fused with a thermo-responsive polypeptide, e.g., an elastin-like polypeptides (ELP) repeats. Methods of using the lectin-ELP fusion ligand for purifying a glycoprotein via affinity precipitation are also provided herewith.
Claims19
An affinity binding ligand comprising a fusion construct comprising lectin fused with a thermal responsive elastin-like protein (ELP), wherein said affinity binding ligand is for purifying a glycoprotein by affinity precipitation.
A method for purifying a glycoprotein of interest by affinity precipitation comprising:
- a) constructing a fusion binding ligand comprising lectin fused with ELP pentapeptide repeats of VPGVG (SEQ ID NO:1);
- b) contacting the fusion binding ligand with said glycoprotein of interest;
- and
- c) isolating and purifying said glycoprotein of interest by affinity precipitation.
Dependencies are inferred from the claim text.