Two-photon probe for detecting copper(ii) ion and quantitative estimation of copper(ii) ion in human tissue using the same
AssigneesKorea University Research and Business Foundation, Sfc Co., Ltd.
InventorBong-Rae CHO
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Abstract
The present disclosure relates to a two-photon probe for in-vivo imaging, having high selectivity for copper(II) ion, and a method for quantitatively estimating copper(II) on (Cu2+) in vivo using the same. The two-phoion probe according to the present disclosure can detect intracellular free copper(H) on for a long period of time with a penetration depth greater than 90 μm in living cells and tissues without the problems of mistargeting and photobleaching and can quantitatively estimate copper(II) on concentration in vivo using the ratio of blue emission intensity at 400-450 nm and red emission intensity at 550-650 nm.
Claims14
A compound represented by [Chemical Formula 1]:
- wherein
- each of R1, R2 and R3, which are identical or different, is independently hydrogen or C1-C10 substituted or unsubstituted alkyl,
- X is —OCH2(CH2OCH2)nCH2OCH3,
- the substituted alkyl is substituted with a substituent selected from a group consisting of halogen, trifluoromethyl, amino, C1-C5 alkyl, C1-C5 alkoxy, hydroxyl, C1-C5 carboxyl, cyano, phenyl and benzyl,
- m is an integer from 1 to 3 and
- n is an integer from 1 to 6.
3-6. (canceled)
A two-photon probe compound for detecting copper(II) ion having a structure represented by [Chemical Formula 1]:
- wherein
- each of R1, R2 and R3, which are identical or different, is independently hydrogen or C1-C10 substituted or unsubstituted alkyl,
- X is —OCH2(CH2OCH2)nCH2OCH3,
- the substituted alkyl is substituted with a substituent selected from a group consisting of halogen, trifluoromethyl, amino, C1-C5 alkyl, C1-C5 alkoxy, hydroxyl, C1-C5 carboxyl, cyano, phenyl and benzyl,
- m is an integer from 1 to 3 and
- n is an integer from 1 to 6.
A method for quantitatively estimating copper(II) ion concentration in vivo using the ratio of emission intensities measured in short wavelength and long wavelength regions as a result of a reaction between a compound of [Chemical Formula 1] and copper(II) ion present in vivo:
- wherein
- each of R1, R2 and R3, which are identical or different, is independently hydrogen or C1-C10 substituted or unsubstituted alkyl,
- X is —OCH2(CH2OCH2)CH2OCH3,
- the substituted alkyl is substituted with a substituent selected from a group consisting of halogen, trifluoromethyl, amino, C1-C5 alkyl, C1-C5 alkoxy, hydroxyl, C1-C5 carboxyl, cyano, phenyl and benzyl and
- m is an integer from 1 to 3 and
- n is an integer from 1 to 6.
Dependencies are inferred from the claim text.