Bolaamphiphilic compounds, compositions and uses thereof

A pharmaceutical composition or a formulation comprising a bolaamphiphile complex, a sub-micron sized vesicle, or nano-sized vesicle;

• wherein the bolaamphiphile complex or nano-sized vesicles comprises one or more bolaamphiphilic compounds and a biologically active compound, wherein the bolaamphiphilic compound is a compound according to formula I:
• HG2-L1-HG1 I
• or a pharmaceutically acceptable salt, solvate, hydrate, prodrug, stereoisomer, tautomer, isotopic variant, or N-oxide thereof, or a combination thereof;
• wherein:
• each HG1 and HG2 is independently a hydrophilic head group;
• and
• L1 is alkylene, alkenyl, heteroalkylene, or heteroalkenyl linker;
• unsubstituted or substituted with C1-C20 alkyl, hydroxyl, or oxo.