Controlling the activity of growth factors, particularly tgf-beta, in vivo
AssigneeUniversity of Florida Research Foundation, Inc.
InventorsAdam Monsalve, Jon P. Dobson
Life of this patent
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Abstract
TGF-β growth factor and its latent complex are conjugated to magnetic micro- or nanoparticles and to magnetic micro- or nanodiscs. By exposing the resulting conjugates to magnetic fields, the TGF-β growth factor can be released from its latent complex in vivo, potentially making it useful in tissue engineering and regenerative medicine. And by exposing a conjugate of TGF-β growth factor and a magnetic particle to a sufficiently strong, radiofrequency magnetic field, the TGF-β growth factor can be denatured and thereby deactivated, potentially making it possible to avoid triggering tumorigenesis, atherosclerosis, fibrotic disease, and cancer.
Claims27
A conjugate of TGF-β and a magnetizable particle.
A conjugate of latent TGF-β complex and a magnetic particle.
A conjugate of a magnetic iron oxide particle that is coated with polyethylene glycol and either
- a. a growth factor;
- or
- b. a latent growth factor complex.
A conjugate of a magnetic iron oxide particle that is coated with polyethylene glycol and either:
- a. a cytokine;
- or
- b. a latent cytokine complex.
A conjugate of TGF-β and a magnetic nanoparticle having a coating selected from the following:
- a. gold;
- b. dextran;
- c. polyethylene glycol;
- or
- d. a biocompatible polymer.
A conjugate of latent TGF-β complex and a magnetic nanoparticle having a coating selected from the following:
- a. gold;
- b. dextran;
- c. polyethylene glycol;
- or
- d. a biocompatible polymer.
A conjugate of latent TGF-β complex and a magnetic spin vortex permalloy micro- or nanodisc, the micro- or nanodisk having gold-coated top and bottom surfaces and an uncoated peripheral edge.
A method of releasing active TGF-β from a latent TGF-β complex in which it is sequestered, comprising:
- a. conjugating the latent TGF-β complex to a magnetic particle;
- and
- b. exposing the resulting conjugate to a magnetic field.
A method of denaturing TGF-β, comprising:
- a. conjugating the TGF-β to a magnetic nanoparticle;
- and
- b. exposing the conjugate to a magnetic field.
A conjugate of a growth factor and a magnetic particle.
Dependencies are inferred from the claim text.