Vectors for transforming mycoplasma hyopneumoniae, transformed m. hyopneumoniae strains, and use thereof
AssigneeHipra Scientific, S.L.U.
InventorsLuis GONZALEZ GONZALEZ, Jaume PIÑOL RIBAS, Jordi MONTANE GIRALT, Maria CAMATS MALET, Enrique QUEROL MURILLO, Marta SITJA ARNAU
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Abstract
The present invention relates to mutant strains of Mycoplasma hyopneumoniae and to a method for preparing them. It also relates to carrier vectors which are used in said method, to vaccine compositions and to vaccination kits comprising the compositions against porcine enzootic pneumonia and other swine diseases. It also relates to the use of M. hyopneumoniae as a host for expressing recombinant proteins and other DNA sequences of interest.
Claims61
A method for preparing a mutant strain of Mycoplasma hyopneumoniae (Mhyo) comprising transforming a strain of Mhyo with a carrier vector comprising at least one exogenous DNA sequence, wherein the at least one exogenous DNA sequence is under the control of a DNA sequence having a degree of identity of at least 80% with a promoter region of Mhyo.
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A replicative plasmid vector, characterized in that it comprises:
- 1) a DNA sequence comprising the oriC region of a strain of Mycoplasma sp., and
- 2) an exogenous DNA sequence comprising a marker gene, and optionally an additional exogenous DNA sequence, each of which are under the control of a DNA sequence having a degree of identity of at least 80% with a promoter region of Mhyo.
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A transposon vector, characterized in that it comprises:
- 1) a DNA sequence coding for a transposase,
- 2) an exogenous DNA sequence comprising a marker gene and,
- 3) optionally, an additional exogenous DNA sequence,
- wherein the DNA sequence coding for a transposase and at least one of the exogenous DNA sequences are under the control of a DNA sequence having a degree of identity of at least 80% with a promoter region of Mhyo.
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A mutant strain of Mhyo characterized in that it comprises at least one exogenous DNA sequence stably incorporated in the genome or in the cytosol thereof.
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Dependencies are inferred from the claim text.