In vitro platform and methods for culturing embryos for implantation
AssigneeCalifornia Institute of Technology
InventorsSamuel Ojosnegros Martos, Carol Readhead, Ayelet Lesman
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Abstract
A mammalian in vitro system for culturing an embryo includes a collagen or fibrin matrix and endometrial and/or stromal cells. The in vitro platforms and methods according to embodiments of the invention allow for in vitro embryonic development (including implantation) prior to transfer of the embryo complex in vivo for further development.
Claims30
A method of culturing a mammalian embryo derived from a first uterus for implantation into a second uterus, the method comprising:
- adding endometrial cells harvested from the first uterus to a collagen or fibrin matrix to form an endometrial-collagen matrix or an endometrial-fibrin matrix;
- and
- adding an isolated embryo derived from the first uterus to the endometrial-collagen matrix or the endometrial-fibrin matrix to form an in vitro embryo culture complex.
A method of culturing a mammalian embryo derived from a first uterus for implantation into a second uterus, the method comprising:
- adding stromal cells harvested from the first uterus to a collagen or fibrinogen suspension to form a stromal-collagen or stromal-fibrinogen suspension;
- polymerizing the stromal-collagen or stromal-fibrinogen suspension to form a stromal-collagen matrix or a stromal-fibrin matrix;
- and
- adding an isolated embryo derived from the first uterus to the stromal-collagen matrix or the stromal-fibrin matrix to form an in vitro embryo culture complex.
A method of culturing an embryo derived from a first uterus for implantation into a second uterus, the method comprising:
- adding endometrial cells and/or stromal cells from the first uterus to a collagen suspension or a fibrinogen suspension to form a cell-collagen suspension or a cell-fibrinogen suspension;
- adding an isolated embryo derived from the first uterus to the cell-collagen or cell-fibrinogen suspension to form an embryo-collagen suspension or an embryo-fibrinogen suspension;
- and
- polymerizing the cell-embryo-collagen suspension or the cell-embryo-fibrinogen suspension to form a cell-embryo-collagen complex or a cell-embryo-fibrin complex.
A system for in vitro culture of a mammalian embryo derived from a first uterus for implantation into a second uterus, the system comprising:
- a collagen or fibrin matrix comprising endometrial cells harvested from the first uterus.
A kit for culturing a mammalian embryo in vitro for implantation into a mammalian uterus, the kit comprising:
- collagen or fibrinogen;
- serum;
- and
- trypsin.
Dependencies are inferred from the claim text.