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OwnerXuan Wang, Lorraine P. Yomano +6inventors
InventorsXUAN WANG, LORRAINE P. YOMANO, JAMES Y. LEE, SEAN W. YORK, HUABAO ZHENG, MICHAEL TODD MULLINNIX, KEELNATHAM T. SHANMUGAM, LONNIE O'NEAL INGRAM
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Four genetic traits have been identified that increase furfural tolerance in microorganisms, such as ethanol-producing Escherichia coli LY180 (strain W derivative). Increased expression of fucO, ucpA or pntAB, and deletion of yqhD were associated with the increase in furfural tolerance. Microorganisms engineered for resistance to furfural were also more resistant to the mixture of inhibitors in hemicellulose hydrolysates, confirming the importance of furfural as an inhibitory component. The combinations of genetic traits disclosed in this application can be applied, generally, to other microorganisms, such as Gram negative and Gram positive bacterial cells, yeast and fungi to increase furfural tolerance in microorganisms used to make industrially useful products.
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An isolated ethanologenic or succinate producing bacterial strain comprising the following genetic modifications:
An isolated bacterial, fungal or yeast cell comprising the following genetic modifications:
A method of increasing furfural and/or 5-hydroxymethylfurfural (5-HMF) resistance in a bacterial, fungal or yeast cell comprising introducing the following genetic modifications to said bacterial, fungal or yeast cell:
An isolated bacterial, fungal or yeast cell comprising a fucO-ucpA construct, said fucO-ucpA construct encoding a lactaldehyde reductase and UcpA oxidoreductase activity.
Dependencies are inferred from the claim text.