Method of manufacturing 1,4-butanediol and microbe
AssigneeShowa Denko K.K.
InventorsHirobumi Aoki, Yuzuru Kokido, Yoko Hashimoto, Tadashi Yoneda
Life of this patent
Estimating the term…
Abstract
A method of manufacturing 1,4-butanediol by an enzyme reaction system via 3-hydroxybutyryl-CoA, crotonyl-CoA and 4-hydroxybutyryl CoA, in this order, using a microbe and/or a culture thereof, wherein the 3-hydroxybutyryl-CoA is an optically active substance, and wherein the microbe includes (1) a gene that codes enoyl-CoA hydratase, (2) a gene that codes vinylacetyl-CoA delta-isomerase, (3) a gene that codes 4-hydroxybutyryl CoA dehydratase, and (4) a gene that codes acyl-CoA reductase whose substrate specificity has an optical selectivity opposite to that of the 3-hydroxybutyryl-CoA.
Claims10
A method of manufacturing 1,4-butanediol by an enzyme reaction system via 3-hydroxybutyryl-CoA, crotonyl-CoA and 4-hydroxybutyryl CoA, in this order, using a microbe and/or a culture thereof,
- wherein the 3-hydroxybutyryl-CoA is an optically active substance, and
- wherein the microbe includes
- (1) a gene that codes enoyl-CoA hydratase,
- (2) a gene that codes vinylacetyl-CoA delta-isomerase,
- (3) a gene that codes 4-hydroxybutyryl CoA dehydratase, and
- (4) a gene that codes acyl-CoA reductase whose substrate specificity has an optical selectivity opposite to that of the 3-hydroxybutyryl-CoA.
A microbe comprising:
- (1) a gene that codes enoyl-CoA hydratase,
- (2) a gene that codes vinylacetyl-CoA delta-isomerase,
- (3) a gene that codes 4-hydroxybutyryl CoA dehydratase,
- (4) a gene that codes acyl-CoA reductase whose substrate specificity to 3-hydroxybutyryl-CoA is optically selective, and
- (5) a gene that codes acetoacetyl-CoA reductase whose substrate specificity to 3-hydroxybutyryl-CoA has an optical selectivity opposite to that of the acyl-CoA reductase.
Dependencies are inferred from the claim text.