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AssigneesSekisui Chemical Co., Ltd., Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.
InventorsMasahiro Furutani, Akihiro Uenishi, Koichiro Iwasa, Stefan Jennewein, Rainer Fischer
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To provide a series of techniques capable of producing isoprene from syngas or the like. Provided is a recombinant cell prepared by introducing a nucleic acid encoding isoprene synthase into a host cell having an isopentenyl diphosphate synthesis ability by a non-mevalonate pathway, wherein the nucleic acid is expressed in the host cell, and the recombinant cell is capable of producing isoprene from at least one C1 compound selected from the group consisting of carbon monoxide, carbon dioxide, formic acid, and methanol. As the host cell, a Clostridium bacterium or a Moorella bacterium is exemplified. Also provided is a method for producing isoprene using the recombinant cell.
1-20. (canceled)
A recombinant cell prepared by introducing a nucleic acid encoding isoprene synthase into a host cell having an isopentenyl diphosphate synthesis ability by a non-mevalonate pathway, wherein the nucleic acid is expressed in the host cell, and the recombinant cell is capable of producing isoprene from at least one C1 compound selected from the group consisting of carbon monoxide, carbon dioxide, formic acid, and methanol.
A recombinant cell prepared by introducing a nucleic acid encoding isoprene synthase into a host cell having a function of synthesizing acetyl CoA from methyltetrahydrofolate, carbon monoxide, and CoA, wherein the nucleic acid is expressed in the host cell, and the recombinant cell is capable of producing isoprene from at least one C1 compound selected from the group consisting of carbon monoxide, carbon dioxide, formic acid, and methanol.
Dependencies are inferred from the claim text.