Solanum lycopersicum plants having non-transgenic alterations in the acs2 gene
AssigneeNunhems B.V.
InventorHendrik Willem Vriezen
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Abstract
The present invention relates to cultivated plant of the species Solanum lycopersicum comprising a acs2 allele having one or more mutations, said mutations resulting in production of a mutant acs2 protein having loss-of-function acs2 protein or reduced function compared to wild type Acs2 protein.
Claims16
A cultivated plant of the species Solanum lycopersicum comprising an acs2 allele having one or more mutations, said mutations resulting in production of a mutant acs2 protein, wherein said mutant acs2 protein has one or more amino acids changed selected from the group consisting of A101T, A101V, A103T, G112R, P118L, V147E, and C265Y in a wild type Acs2 protein, said protein having at least 85% amino acid sequence identity to SEQ ID NO:
- 1.
The cultivated plant according to any one of the preceding claims, wherein said mutation or mutations result in the tomato fruits requiring significantly more days to reach the bad stage compared to Solanum lycopersicum being homozygous for the wild type Acs2 allele encoding said wild type Acs2 protein.
The plant according to any one of the preceding claims, wherein the plant is an F1 hybrid plant.
The plant according to any one of the preceding claims, wherein the acs2 allele having one or more mutation, is in homozygous form.
The cultivated plant according to any one of the preceding claims, wherein the plant additionally comprises an acs4 allele having one or more mutations, said mutations in said acs4 allele resulting in production of a mutant acs4 protein having loss-of-function or reduced function compared to wild type Acs4 protein and having at least 85% amino acid sequence identity to the Acs4 amino acid sequence provided in GenBank Accession number M63490.1;
- and optionally, wherein said mutant acs4 allele is obtained from mutant 2477 and/or mutant 4043 and/or mutant 4222 and/or mutant 4303 and/or mutant 4691 and/or mutant 5251.
Seed from which a plant according to any one of the preceding claims can be grown.
Dependencies are inferred from the claim text.