US 2015/0275308 A1 · MONITORING DIFFUSE LARGE B-CELL LYMPHOMA FROM PERIPHERAL BLOOD SAMPLES

Abstract

The invention is directed to sequencing-based methods for monitoring a minimal residual disease of a diffuse large B cell lymphoma (DLBCL) by one or more clonotypes correlated with the disorder. In some embodiments, such methods comprise the following steps: (a) obtaining a sample of peripheral blood from the patient; (b) amplifying molecules of nucleic acid from the sample, the molecules of nucleic acid comprising recombined DNA sequences from immunoglobulin genes; (c) sequencing the amplified molecules of nucleic acid to form a clonotype profile; and (d) determining from the clonotype profile a presence, absence and/or level of one or more patient-specific clonotypes correlated with the DLBCL and phylogenic clonotypes thereof.

Claims19

Dependent claims

A method of monitoring diffuse large B-cell lymphoma (DLBCL) disease in a patient, the method comprising the steps of:

(a) determining one or more patient-specific clonotypes correlated with the DLBCL from a diagnostic sample;
(b) obtaining a peripheral blood sample from the patient comprising B-cells and/or cell-free nucleic acids;
(c) amplifying nucleic acid molecules from the B-cells and/or the cell-free nucleic acids of the peripheral blood sample, the nucleic acid molecules comprising recombined DNA sequences from immunoglobulin genes or nucleic acids transcribed therefrom;
(d) sequencing the amplified nucleic acid molecules to form a clonotype profile;
and
(e) determining from the clonotype profile a presence, absence and/or level of the one or more patient-specific clonotypes correlated with the DLBCL, including previously phylogenic clonotypes thereof.

3 more elements

Dependencies are inferred from the claim text.

Monitoring diffuse large b-cell lymphoma from peripheral blood samples

Life of this patent

Monitoring diffuse large b-cell lymphoma from peripheral blood samples

Life of this patent

Abstract

Claims19