Method of manufacturing 1,4-butanediol and microbe
AssigneeShowa Denko K.K.
InventorsHirobumi Aoki, Yuzuru Kokido, Yoko Hashimoto, Tadashi Yoneda
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Abstract
A method of manufacturing 1,4-butanediol, using a microbe and/or a culture thereof, by an enzyme reaction system that uses acetoacetyl-CoA reductase and enoyl-CoA hydratase, via acetoacetyl-CoA, 3-hydroxybutyryl-CoA and crotonyl-CoA in this order, wherein each of the acetoacetyl-CoA reductase and the enoyl-CoA hydratase is specific to a stereoisomer of 3-hydroxybutyryl-CoA.
Claims13
A method of manufacturing 1,4-butanediol, using a microbe and/or a culture thereof, by an enzyme reaction system that uses acetoacetyl-CoA reductase and enoyl-CoA hydratase, via acetoacetyl-CoA, 3-hydroxybutyryl-CoA and crotonyl-CoA in this order,
- wherein each of the acetoacetyl-CoA reductase and the enoyl-CoA hydratase is specific to a stereoisomer of 3-hydroxybutyryl-CoA.
A microbe that manufactures 1,4-butanediol by an enzyme reaction system that uses acetoacetyl-CoA reductase and enoyl-CoA hydratase, via acetoacetyl-CoA, 3-hydroxybutyryl-CoA and crotonyl-CoA in this order,
- wherein each of the acetoacetyl-CoA reductase and the enoyl-CoA hydratase is specific to a stereoisomer of 3-hydroxybutyryl-CoA.
Dependencies are inferred from the claim text.