Genetically engineered yeast
AssigneeTechnical University of Denmark
InventorsIrina Borodina, Kanchana Rueksomtawin Kildegaard, Jochen Förster, Fredrik Öberg
Life of this patent
Estimating the term…
Abstract
A genetically modified Saccharomyces cerevisiae including an active fermentation pathway producing 3-HP expresses an exogenous gene expressing the aminotransferase YhxA from Bacillus cereus AH1272 catalysing a transamination reaction between beta-alanine and pyruvate to produce malonate semialdehyde. The yeast may also express a 3-hydroxyisobutyrate dehydrogenase (HIBADH) and a 3-hydroxypropanoate dehydrogenase (3-HPDH) and aspartate 1-decarboxylase. Additionally the yeast may express pyruvate carboxylase and aspartate aminotransferase.
Claims15
A genetically modified yeast cell comprising an active fermentation pathway producing 3-HP, wherein the cell comprises and expresses an exogenous gene coding for the production of an enzyme having at least 80% identity with SEQ ID NO:
- 1 and catalysing a transamination reaction between beta-alanine and pyruvate to produce malonate semialdehyde.
A method for the production of 3HP comprising culturing a genetically modified yeast cell comprising an active fermentation pathway producing 3-HP, wherein the cell comprises and expresses an exogenous gene coding for the production of an enzyme having at least 80% identity with SEQ ID NO:
- 1 and catalysing a transamination reaction between beta-alanine and pyruvate to produce malonate semialdehyde, and recovering 3HP from the culture.
Dependencies are inferred from the claim text.