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US 2015/0267226 A1Published application
Recombinant microorganism with ability to produce glycerol, 3-hp, or acrylic acid and method of producing glycerol, 3-hp, or acrylic acid by using the same
AssigneeSamsung Electronics Co., Ltd.
InventorsWonseok Jung, Eunyoung Kim, Jinho Kang, Nagjong Kim
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Abstract
A recombinant microorganism having the ability to produce glycerol 3-HP, or acrylic acid, in which glycerol is produced from dihydroxyacetone phosphate (DHAP) via dihydroxyacetone (DHA) in a biosynthetic pathway, and a method of producing glycerol, 3-hydroxypropioninc acid (3-HP), or acrylic acid by using the recombinant microorganism.
Claims20
A recombinant microorganism comprising:
- a polynucleotide encoding dihydroxyacetone phosphate phosphatase (DHAPP) that catalyzes the conversion of dihydroxyacetone phosphate (DHAP) into dihydroxyacetone (DHA);
- and
- a polynucleotide encoding glycerol dehydrogenase (GLDH) that catalyzes the conversion of DHA into glycerol.
A method of making a microorganism that produces glycerol, the method comprising introducing into a microorganism that does not produce glycerol:
- a polynucleotide encoding dihydroxyacetone phosphate phosphatase (DHAPP) that catalyzes the conversion of dihydroxyacetone phosphate (DHAP) into dihydroxyacetone (DHA);
- and
- a polynucleotide encoding glycerol dehydrogenase (GLDH) that catalyzes the conversion of DHA into glycerol;
- thereby providing a microorganism that produces glycerol,
- wherein the microorganism belongs to the genus Escherichia.
2 more elements
Dependencies are inferred from the claim text.