METHODS OF PRODUCING 6-CARBON CHEMICALS VIA CoA-DEPENDENT CARBON CHAIN ELONGATION ASSOCIATED WITH CARBON STORAGE
AssigneeInvista North America S.A R.L.
InventorsAdriana Leonora Botes, Alex Van Eck Conradie
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Abstract
This document describes biochemical pathways for producing adipic acid, caprolactam, 6-aminohexanoic acid, 6-hydroxyhexanoic acid, hexamethylenediamine or 1,6-hexanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl groups, in a C6; backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on CoA-dependent elongation enzymes or analogues enzymes associated with the carbon storage pathways from polyhydroxyalkanoate accumulating bacteria.
Claims34
-40. (canceled)
A recombinant host comprising at least one exogenous nucleic acid encoding:
- (i) either a β-ketothiolase or an acetyl-CoA carboxylase and an acetoacetyl-CoA synthase, (ii) a 3-hydroxyacyl-CoA dehydrogenase or a 3-oxoacyl-CoA reductase, (iii) an enoyl-CoA hydratase, and (iv) a trans-2-enoyl-CoA reductase, wherein the host produces hexanoyl-CoA.
Dependencies are inferred from the claim text.