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AssigneeBangladesh Jute Research Institute
InventorsMaqsudul Alam, Mohammed S. Islam, Mohammed M. Hossen, Mohammed S. Haque, Mohammed M. Alam
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The present invention discloses isolated polynucleotide encoding enzymes, derived from the fungus Macrophomina phaseolina (“M. phaseolina”), responsible for degrading pectin, and it comprises and/or consists of nucleotide sequences set forth in SEQ. ID Nos. 2, 5, 8, 11, 14, 17, 20, 23, 26, 29, 32, 35, 38, 41, 44, 47, 50, 53, 56, 59, 62, 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58 and 61, or the complement of such sequences. The present invention also relates to isolated polypeptide encoded by the polynucleotide sequences set forth in SEQ ID Nos. 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60 and 63; a recombinant gene construct comprising the polynucleotide; a transformant and a transgenic fungus comprising the recombinant gene construct, with or having enhanced production of pectin degrading enzyme. The polypeptide of the invention can be used for, amongst other things, manufactured fruit juice, textile products, pulp and paper, coffee, tea and oil extraction and pectic waste water treatment.
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An isolated polynucleotide sequence which is at least 60% identical to the nucleotide sequence set forth in SEQ ID No. 2, 5, 8, 11, 14, 17, 20, 23, 26, 29, 32, 35, 38, 41, 44, 47, 50, 53, 56, 59, or 62, or any combination thereof or the complement thereof.
A nucleic acid probe that hybridizes under medium stringency conditions to an isolated polynucleotide sequence which is at least 60% identical to the nucleotide sequence set forth in SEQ ID No. 2, 5, 8, 11, 14, 17, 20, 23, 26, 29, 32, 35, 38, 41, 44, 47, 50, 53, 56, 59, or 62,
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A recombinant construct comprising the isolated polynucleotide sequence of claim.
13-18. (canceled)
An isolated polypeptide comprising an amino acid sequence which is at least 60% identical to an amino acid sequence selected from the group consisting of SEQ ID Nos. 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60 and 63, or any combination thereof, and displays the catalytic activity of an enzyme selected from the group consisting of pectate lyase, pectate lyse C, pectinesterase and rhamnogalacturonase, or any combination thereof.
21-30. (canceled)
Dependencies are inferred from the claim text.