Loading patent…
AssigneesThe United States of America as represented by the Secretary of Agriculture, University of Conneticut
InventorsManuel V. Borca, Guillermo R. Risatti, Lauren G. Holinka-Patterson
Estimating the term…
Controlling Classical Swine Fever Virus (CSFV) involves either prophylactic vaccination or non-vaccination and elimination of infected herds depending on the epidemiological situation. Marker vaccines allowing distinction between naturally infected from vaccinated swine could complement “stamping out” measures. Previously, we reported the development of FlagT4v, a double antigenic marker live attenuated CSFV strain. FlagT4v was later shown as not to be completely stable in terms of its attenuation when assessed in a reversion to virulence protocol. We have developed a modified version of the FlagT4v where changes in the codon usage of genomic areas encoding for Flag and T4 were introduced to rectify the reversion to the virulent genotype. The new virus, FlagT4-mFT-Gv, possesses the same amino acid sequence as FlagT4v except for one substitution, Asparagine is replaced by Glycine at position 852 of the CSFV polypeptide. FlagT4-mFT-Gv protected swine against challenge with Brescia virulent virus at 21 days post vaccination.
A recombinant classical swine fever virus (CSFV) mutant, FlagT4-mFT-Gv, comprising cDNA (SEQ ID NO:1) encoding a mutation in CSFV E1 glycoprotein wherein the mutation comprises a 20 mer insertion, said mutation being identical to the mutant FlagT4, wherein said recombinant CSFV mutants, FlagT4-mFT-Gv and FlagT4, are live attenuated CSFVs and said FLAG™ epitope carried by said live attenuated CSFVs is capable of being recognized by monoclonal antibodies specifically binding to said FLAG™ epitope, which serves as a positive marker for said attenuated CSFV mutant, wherein the nucleotides 2435-2488 of said cDNA (SEQ ID NO:1) of FlagT4-mFT-Gv have been changed and differ entirely from nucleotides 2435-2488 of the cDNA sequence (SEQ ID NO:3) of FlagT4 and said nucleotides encode the same amino acids in positions 688-705 of SEQ ID NO:2 and SEQ ID NO:4 of FlagT4-mFTGv and FlagT4, respectively.
Dependencies are inferred from the claim text.