Methods for detecting lp-pla2 activity and inhibition of lp-pla2 activity
AssigneeGlaxo Group Limited
InventorsYaping SHOU, Yin Fai Amy SIU, George T. WALKER
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Abstract
This invention relates to methods for determining the activity of Lp-PLA2 in at least one sample from an animal. The invention also relates to methods for determining the inhibition of Lp-PLA2 activity in samples from animals that are administered an Lp-PLA2 inhibitor.
Claims29
A colorimetric or fluorometric method of determining the percentage of inhibition of Lp-PLA2 activity, the method comprising:
- colorimetrically or fluorometrically detecting Lp-PLA2 activity from a first blood sample that is taken from the subject at a first time and diluted less than 33 times;
- colorimetrically or fluorometrically detecting Lp-PLA2 activity from a second blood sample that is taken from the subject at a second time one hour or more after the first time and diluted less than 33 times;
- and
- detecting greater than 30% inhibition of Lp-PLA2 activity between the first and second blood samples by comparing the activity of the Lp-PLA2 between the first and second blood samples, indicating that the subject has taken a drug that inhibits Lp-PLA2.
A method of clinically monitoring a patient to assess the efficacy of an inhibitor of lipoprotein-associated phospholipase A2 (Lp-PLA2), the method comprising:
- receiving a sample from an animal that has been administered an Lp-PLA2 inhibitor;
- and
- colorimetrically or fluorometrically detecting greater than 30 percent inhibition of Lp-PLA2 from the sample by contacting the sample with a solution comprising a substrate for Lp-PLA2.
A method of determining inhibition of lipoprotein-associated phospholipase A2 (Lp-PLA2) enzyme activity from a sample, the method comprising:
- preparing a solution comprising a substrate for Lp-PLA2;
- contacting a sample from an animal that has been administered an Lp-PLA2 inhibitor, wherein the sample is diluted less than a 33 fold by contact with the solution;
- and
- colorimetrically or fluorometrically detecting Lp-PLA2 activity.
Dependencies are inferred from the claim text.