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US 2015/0247801 A1Published application
Spatial Positioning of Spectrally Labeled Beads
AssigneeLife Technologies Corporation
InventorsStephen Empedocles, Andrew WATSON, Jian Jin
Life of this patent
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Abstract
Devices, systems, kits, and methods for detecting and/or identifying a plurality of spectrally labeled bodies well-suited for performing multiplexed assays. By spectrally labeling the beads with materials which generate identifiable spectra, a plurality of beads may be identified within the fluid. Reading of the beads is facilitated by restraining the beads in arrays, and/or using a focused laser.
Claims22
1-16. (canceled)
A method comprising:
- restraining a plurality of spectrally labeled bodies with affinity binding so as to define a multiplexed array, wherein the spectrally labeled bodies are spatially resolved and comprise a plurality of fluorescent labels used to encode discrete and different emission spectra wherein each emission spectrum has a plurality of signals at differing wavelengths;
- directing an image of the multiplexed array of bodies onto a sensor to sense spectra generated by the bodies;
- and
- identifying the bodies from the spectra sensed by the sensor.
1 more element
Dependencies are inferred from the claim text.