Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of isoleucyl trna synthetases
AssigneesaTyr Pharma, Inc., Pangu BioPharma Limited
InventorsLeslie Ann Greene, Kyle P. Chiang, Fei Hong, Alain P. Vasserot, Wing-Sze Lo, Jeffry D. Watkins, Cheryl L. Quinn, John D. Mendlein
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Abstract
Provided are compositions comprising newly identified protein fragments of aminoacyl-tRNA synthetases, polynucleotides that encode them and complements thereof, related agents, and methods of use thereof in diagnostic, drug discovery, research, and therapeutic applications.
Claims15
1-125. (canceled)
A therapeutic composition, comprising an isolated aminoacyl-tRNA synthetase (AARS) polypeptide of about 100 to about 750 amino acids in length and comprising an amino acid sequence that is at least 80%, 85%, 90%, 95%, 98%, or 100% identical to a sequence in any of Table(s) 1-3, or Table(s) 4-6, or Table(s) 7-9, wherein the polypeptide has a solubility of at least about 5 mg/mL, and wherein the composition has a purity of at least about 95% on a protein basis and less than about 10 EU endotoxin/mg protein.
An isolated aminoacyl-tRNA synthetase (AARS) polypeptide of about 100 to about 750 amino acids in length and comprising an amino acid sequence that is at least 80%, 85%, 90%, 95%, 98%, or 100% identical to a sequence in any of Table(s) 1-3, or Table(s) 4-6, or Table(s) 7-9.
A method of determining presence or levels of an AARS polypeptide in a sample, comprising contacting the sample with one or more binding agents that specifically bind to an AARS polypeptide as set forth in any of Table(s) 1-3, or Table(s) 4-6, or Table(s) 7-9, detecting the presence or absence of the binding agent, and thereby determining the presence or levels of the AARS polypeptide.
An engineered full length aminoacyl-tRNA synthetase (AARS) protein comprising a heterologous proteolysis site to enable the proteolytic generation of an AARS polypeptide as set forth in any of Table(s) 1-3, or Table(s) 4-6, or Table(s) 7-9.
A composition, comprising a binding agent that specifically binds to an isolated aminoacyl-tRNA synthetase (AARS) polypeptide fragment as set forth in any of Table(s) 1-3, or Table(s) 4-6, or Table(s) 7-9, wherein the binding agent has an affinity of at least about 1 nM for the polypeptide fragment.
Dependencies are inferred from the claim text.