Monodisperse microdroplet generation and stopping without coalescence

An apparatus for monodispersed microdroplet generation and trapping of a sample wherein the sample is in an aqueous solution, comprising:

• a microfluidic microchip;
• a flow channel in said microchip;
• a source of the sample in an aqueous solution, a source fluid and magnetic particles wherein the sample adheres to said magnetic particles, said source connected to said microfluidic flow channel, said source including a pump or pressure source;
• a droplet maker that generates microdroplets containing the sample in an aqueous solution, said source fluid and said magnetic particles, said droplet maker connected to said microfluidic flow channel and connected to said source of the sample, said source fluid and said magnetic particles, wherein said droplet maker includes a T-junction connected to said microfluidic flow channel and connected to said source of the sample, said source fluid and said magnetic particles producing microdroplets containing the sample, said source fluid and said magnetic particles;
• a carrier fluid in said microfluidic flow channel, said carrier fluid introduced to said microfluidic flow channel by
• a source of carrier fluid wherein said source of carrier fluid is a source of oil carrier fluid and wherein said source fluid is water wherein said carrier fluid in said flow channel is oil producing microdroplets of water source fluid in oil carrier fluid in said flow channel, said source of carrier fluid including a pump or pressure source;
• a trapping section in said microfluidic flow channel including a magnet;
• and
• a valve system connected to said microfluidic flow channel and said carrier fluid that controls flow of said carrier fluid and said microdroplets containing the sample, said source fluid and said magnetic particles that enables trapping of said microdroplets in said trapping section.

An apparatus for monodispersed microdroplet generation and trapping of a sample wherein the sample is in an aqueous solution, comprising:

• a microchip;
• a microfluidic flow channel in said microchip;
• a source of the sample in an aqueous solution, a source fluid and non magnetic particles wherein the sample adheres to said non magnetic particles, said source connected to said microfluidic flow channel, said source including a pump or pressure source;
• a droplet maker that generates microdroplets containing the sample in an aqueous solution, said source fluid and said non magnetic particles, said droplet maker connected to said microfluidic flow channel and connected to said source of the sample, said source fluid and said non magnetic particles, wherein said droplet maker includes a T-junction connected to said microfluidic flow channel and connected to said source of the sample, said source fluid and said non magnetic particles producing microdroplets containing the sample, said source fluid and said non magnetic particles;
• a carrier fluid in said microfluidic flow channel, said carrier fluid introduced to said microfluidic flow channel by
• a source of carrier fluid wherein said source of carrier fluid is a source of water carrier fluid and wherein said source fluid is oil, wherein said carrier fluid in said flow channel is water producing microdroplets of oil source fluid, the sample, and said non magnetic particles in water carrier fluid in said microfluidic flow channel, said source of carrier fluid including a pump or pressure source;
• a trapping section in said microfluidic flow channel;
• and
• a valve system connected to said carrier fluid that controls flow of said carrier fluid to stop said non magnetic particles and said microdroplets in said trapping section and enables trapping of said microdroplets and said non magnetic particles in said trapping section.