Microorganism having carbon dioxide fixation cycle introduced thereinto

A method of producing acetyl-CoA, comprising:

• culturing an acetyl-CoA producing microorganism comprising an acetyl-CoA production cycle obtained by imparting at least one enzymatic activity selected from the group consisting of malate thiokinase, malyl-CoA lyase, glyoxylate carboligase, 2-hydroxy-3-oxopropionate reductase, and hydroxypyruvate reductase, to a microorganism that does not have any of the following (a), (b), (c), (d) or (e):
• (a) a carbon dioxide fixation cycle including an enzymatic reaction from malonyl-CoA to malonate semialdehyde or 3-hydroxypropionate;
• (b) a carbon dioxide fixation cycle including an enzymatic reaction from acetyl-CoA and CO2 to pyruvate;
• (c) a carbon dioxide fixation cycle including an enzymatic reaction from crotonyl-CoA and CO2 to ethylmalonyl-CoA or glutaconyl-CoA;
• (d) a carbon dioxide fixation cycle including an enzymatic reaction from CO2 to formate;
• or
• (e) at least one selected from the group consisting of malate thiokinase and malyl-CoA lyase,
• the acetyl-CoA producing microoranism being obtained without imparting any of (a), (b), (c), or (d) to the microorganism, or such that the microorganism exhibits none of the functions of (a), (b), (c), or (d) even if one or more of (a), (b), (c), or (d) is imparted thereto;
• and
• supplying at least one selected from the group consisting of a carbonate ion or a bicarbonate ion with a total supply amount of 150 mmol/L or more, carbon dioxide gas with an average bubble diameter of 100 μm or more, and sodium sulfite with a total supply amount of from 0.01 g/l to 50 g/L, to a culture medium used for the culturing.

A microorganism belonging to the genus Aspergillus or the genus Cupriavidus obtained by imparting at least one enzymatic activity selected from the group consisting of malate thiokinase and malyl-CoA lyase, to a microorganism that does not have any of the following (a2), (b2), (c2), (d2) or (e2):

• (a2) a carbon dioxide fixation cycle including an enzymatic reaction from malonyl-CoA to malonate semialdehyde or 3-hydroxypropionate;
• (b2) a carbon dioxide fixation cycle including an enzymatic reaction from acetyl-CoA and CO2 to pyruvate;
• (c2) a carbon dioxide fixation cycle including an enzymatic reaction from crotonyl-CoA and CO2 to ethylmalonyl-CoA or glutaconyl-CoA;
• (d2) a carbon dioxide fixation cycle including an enzymatic reaction from CO2 to formate;
• or
• (e2) at least one selected from the group consisting of malate thiokinase and malyl-CoA lyase,
• the microorganism belonging to the genus Aspergillus or the genus Cupriavidus being obtained without imparting any of (a2), (b2), (c2), or (d2) to the microorganism, or such that the microorganism exhibits none of the functions of (a2), (b2), (c2), or (d2) even if one or more of (a2), (b2), (c2), or (d2) is imparted thereto.