Biotechnological production of cyanophycin dipeptides
AssigneeWestfäLische Wilhelms-UniversitäT MüNster
InventorsAhmed SALLAM, Alexander Steinbüchel
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Abstract
The present invention relates to a process for the enzymatic production of a dipeptide composition from a cyanophycin (CGP) or CGP-like polymer preparation by degrading the polymer preparation with an CGPase, a CGPase particularly adapted for said process, and the use of cyanophycin (CGP) or CGP-like polymers or fragments thereof, notably a dipeptide composition obtained by the process as defined above, as pharmaceutical composition, medicament, or as food or feed substitute.
Claims15
A CGPase prepared by a process comprising degrading a CGP or CGP-like polymer with an CGPase from P. alcaligenes, which has a molecular weight of about 45 kDa, an optimum temperature of about 50° C. and an optimum pH range of 7 to 8.5 and degrades CGP into β-dipeptides.
A composition comprising isolated β-dipeptides or an isolated β-dipeptide mixture obtained from a cyanophycin (CGP) or a CGP-like polymer being peptidic structures essentially comprised of one or more β-dipeptide units composed of two amino acids selected from aspartic acid, arginine, lysine, glutamic acid, citrulline, ornithine and canevanine, by a proteolytic process comprising degrading a CGP or CGP-like polymer with an CGPase from P. alcaligenes, which has a molecular weight of about 45 kDa, an optimum temperature of about 50° C. and an optimum pH range of 7 to 8.5 and degrades CGP into β-dipeptides.
Dependencies are inferred from the claim text.