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US 2015/0225709 A1Published application
Methods for the manufacture of proteolytically processed polypeptides
AssigneeSyntaxin Limited
InventorAndreas Rummel
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Abstract
The present invention relates to a novel proteolytically active polypeptide and various uses of the polypeptide (and others) in screening and manufacturing methods.
Claims25
A proteolytically active truncation of a precursor polypeptide, wherein said precursor polypeptide consists of a polypeptide having at least 90% sequence identity with SEQ ID NO:
- 2, wherein said proteolytically active truncation lacks one or more amino acid residues N-terminal to amino acid position 249 of SEQ ID NO:
- 2, and wherein said proteolytically active truncation is capable of hydrolysing botulinum or tetanus neurotoxin to produce di-chain botulinum or di-chain tetanus neurotoxin.
(canceled)
(canceled)
A method for the manufacture of a proteolytically processed polypeptide, comprising:
- contacting a first polypeptide, said first polypeptide being Lys-C or a homolog thereof having at least 60% sequence identity thereto with a second polypeptide, said second polypeptide being susceptible to proteolysis by said first polypeptide;
- wherein said contacting results in proteolytic processing of said second polypeptide into at least two cleavage products;
- wherein the second polypeptide is a single-chain botulinum neurotoxin serotype A (BoNT/A);
- and
- wherein said first polypeptide hydrolyses the single chain botulinum neurotoxin serotype A (BoNT/A) to produce a di-chain botulinum neurotoxin serotype A (BoNT/A).
2 more elements
Dependencies are inferred from the claim text.