Human ebola virus species and compositions and methods thereof
AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Serv
InventorsJonathan S. Towner, Stuart T. Nichol, James A. Comer, Thomas G. Ksiazek, Pierre E. Rollin
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Abstract
Compositions and methods including and related to the Ebola Bundibugyo virus (EboBun) are provided. Compositions are provided that are operable as immunogens to elicit and immune response or protection from EboBun challenge in a subject such as a primate. Inventive methods are directed to detection and treatment of EboBun infection.
Claims18
A method for detecting the presence or absence of Ebola Bundibugyo comprising a nucleic acid molecule comprising a nucleotide sequence as set forth in SEQ ID NO:
- 1, said method comprising:
- (a) contacting a biological sample with an agent that selectively binds to Ebola Bundibugyo or the nucleic acid molecule derived therefrom;
- and
- (b) detecting the presence or absence of the compound binding to said Ebola Bundibugyo or the nucleic acid molecule derived therefrom in said sample.
A vaccine comprising:
- a) a protein consisting of an amino acid sequence of
- 5 to 250 contiguous amino acid residues of the amino acid sequence of SEQ ID NOS:
- 5 or 18 (VP24);
- 5 to 280 contiguous residues of the amino acid sequence of SEQ ID NOS:
- 6 or 17 (VP30);
- 5 to 320 contiguous residues of the amino acid sequence of SEQ ID NOS:
- 8 or 13 (VP40);
- 5 to 340 contiguous residues of the amino acid sequence of SEQ ID NOS:
- 7 or 12 (VP35);
- 5 to 370 contiguous residues of the amino acid sequence of SEQ ID NOS:
- 4 or 15 (SGP);
- 5 to 370 contiguous residues of the amino acid sequence of SEQ ID NOS:
- 59 or 16 (SSGP);
- 5 to 670 contiguous residues of the amino acid sequence of SEQ ID NOS:
- 9 or 14 (GP);
- 5 to 730 contiguous residues of the amino acid sequence of SEQ ID NOS:
- 3 or 11 (NP);
- or
- 5 to 2200 contiguous residues of the amino acid sequence of SEQ ID NOS:
- 2 or 19 (L);
- and
- an adjuvant;
- or
- b) a plasmid comprising a nucleic acid molecule encoding a protein selected from the group consisting of SEQ ID NOS:
- 2, 3, 4, 5, 6, 7, 8, 9, 11, 12, 13, 14, 15, 16, 17, 18, 19, and 59.
An attenuated hEbola virus comprising a nucleic acid molecule comprising a nucleotide sequence of:
- a) a nucleotide sequence set forth in SEQ ID NO:
- 1;
- b) a nucleotide sequence hybridizing under stringent conditions to SEQ ID NO:
- 1;
- or
- c) a nucleotide sequence of at least 70%-99% identity to SEQ ID NO:
- 1, with the proviso that said nucleotide sequence is not SEQ ID NO:
- 20.
Dependencies are inferred from the claim text.