Tall fescue endophyte isolate 647
AssigneeDLF Trifolium A/S
InventorsNiels Roulund, Anne Mette Dahl Jensen
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Abstract
This disclosure provides to a new tall fescue grass endophyte (Neotyphodium coenophialum) referred to as 647, as well as to synthetic combinations of the 647 endophyte and tall fescue grass plant or tall fescue grass cultivar. This new endophyte does not produce detectable ergovaline or any other ergopeptide alkaloids which are known to be harmful to grazing livestock. Thus, the 647 endophyte can be used in combination with forage grasses. In addition, the 647 endophyte provides increased green matter yield and improved resistance to black beetles (Heteronychus arator) and other pests. Thus, the 647 endophyte can be used in combination with grasses susceptible to infection or infestation with black beetles.
Claims24
An axenic culture of an endophyte deposited with The Centraalbureau voor Schimmelcultures (CBS) as Deposit No. CBS 133163.
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A method to screen for an Neotyphodium coenophialum endophyte that is phenotypically similar to Neotyphodium coenophialum endophyte 647 comprising the steps:
- i) providing an isolated Neotyphodium coenophialum endophyte to be tested and extracting genomic DNA;
- ii) forming a preparation comprising:
- one or more pairs of oligonucleotide probes adapted to anneal to genomic DNA comprising microsatellite nucleotide sequences selected from the group consisting of B1, B4, B9, B10, B11, NCESTA1AG05, NCESTA1DH04, NCESTA1FH03, NCESTA1IC04, NCESTA1GA07, NCESTA1AG12, NCESTA1FC04, and NCESTA1DB06;
- a thermostable DNA polymerase, deoxynucleotide triphosphates and co-factors;
- i) providing polymerase chain reaction conditions sufficient to amplify all or part of said genomic microsatellite DNA;
- ii) analysing the amplified products of said polymerase chain reaction for the presence of genomic microsatellite DNA as defined in ii) above;
- and optionally
- v) comparing the amplified product with one or more Neotyphodium coenophialum endophytes including Neotyphodium coenophialum endophyte 647.
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Dependencies are inferred from the claim text.