Xylose Isomerases and Their Uses
AssigneeBP Corporation North America Inc.
InventorsDavid Neal Nunn, JR., Peter Luginbuhl, Ling Li, Adam Martin Burja, Jon Peter Flash Bartnek
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Abstract
This disclosure relates to novel xylose isomerases and their uses, particularly in fermentation processes that employ xylose-containing media.
Claims41
1-4. (canceled)
A recombinant polypeptide comprising an amino acid sequence having at least 95%, 97% or 98% sequence identity to amino acids 2-377 of SEQ ID NO:96.
A recombinant polypeptide comprising an amino acid sequence having at least 80%, 85%, 90%, 93% or 95% sequence identity to amino acids 2-377 of SEQ ID NO:96 and further comprises the amino acid sequence of (a) SEQ ID NO:212 or SEQ ID NO:213 and/or (b) SEQ ID NO:214.
9-37. (canceled)
An isolated nucleic acid which encodes a polypeptide comprising an amino acid sequence having at least 95%, 97% or 98% sequence identity to amino acids 2-377 of SEQ ID NO:96.
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An isolated nucleic acid comprising a nucleotide sequence having at least 40%, at least 50%, at least 60%, least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 93%, at least 95%, at least 96%, at least 98%, or at least 99% sequence identity, or having 100% sequence identity, to the nucleotide sequence of any one of SEQ ID NO:
- 95 or to a portion thereof encoding a xylose isomerase catalytic or dimerization domain.
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A recombinant cell engineered to express the polypeptide comprising an amino acid sequence having at least 95%, 97% or 98% sequence identity to amino acids 2-377 of SEQ ID NO:96.
62-69. (canceled)
A method for producing a fermentation product, comprising culturing the recombinant cell engineered to express a polypeptide comprising an amino acid sequence having at least 95%, 97% or 98% sequence identity to amino acids 2-377 of SEQ ID NO:96. in medium containing xylose under conditions in which the fermentation product is expressed.
78-81. (canceled)
Dependencies are inferred from the claim text.