Methods and compositions for identifying jc virus
AssigneeThe United States of America, as Represented by th e Secretary,Department of Health and Human Servic
InventorsEugene Major, Caroline Ryschkewitsch
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Abstract
The disclosure relates to methods and compositions for detecting the presence of Prototype and/or Archetype JC virus in a biological sample from a subject. In some embodiments, the methods include amplifying and detecting a first nucleic acid sequence unique to Archetype JC virus in a biological sample, and a second nucleic acid sequence common to both Archetype and Prototype JC virus in the biological sample. In several embodiments, the methods can be used to identify JC virus in a biological sample from a subject at risk for progressive multifocal leukoencephalopathy. Compositions and kits for use in the disclosed methods are also provided.
Claims51
A method of detecting the presence of Prototype and/or Archetype JC virus in a biological sample from a subject, comprising:
- amplifying a first nucleic acid molecule from the biological sample using a first oligonucleotide primer pair comprising a forward oligonucleotide primer comprising at least 12 consecutive nucleotides of the nucleotide sequence set forth as nucleotides 93-138 of SEQ ID NO:
- 1 and a reverse oligonucleotide primer comprising at least 12 consecutive nucleotides complementary to nucleotides 159-206 of SEQ ID NO:
- 1;
- amplifying a second nucleic acid molecule from the biological sample comprising a nucleic acid sequence of the JC virus genome present in both Archetype and Prototype JC virus;
- and
- determining whether the first and second nucleic acid molecule have been amplified;
- wherein
- amplification of the first nucleic acid molecule determines the presence of Archetype JC virus in the biological sample;
- and
- amplification of more of the first nucleic acid molecule than the second nucleic acid molecule determines the presence of Prototype JC virus in the biological sample, thereby detecting the presence of Prototype and/or Archetype JC virus in the biological sample.
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A composition, comprising a first oligonucleotide primer pair comprising a forward oligonucleotide primer comprising at least 12 consecutive nucleotides of the nucleotide sequence set forth as nucleotides 93 to 138 of SEQ ID NO:
- 1 and a reverse oligonucleotide primer comprising at least 12 consecutive nucleotides complementary to nucleotides 159 to 206 of SEQ ID NO:
- 1.
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Dependencies are inferred from the claim text.