US 2015/0176086 A1 · PNA Probes, Probe Sets, Methods and Kits Pertaining to the Detection of Candida

Abstract

This invention is related to novel PNA probes, probe sets, methods and kits pertaining to the detection of one or more species of Candida yeast. Non-limiting examples of probing nucleobase sequences that can be used for the probes of this invention can be selected from the group consisting of: AGA-GAG-CAG-CAT-GCA (Seq. Id. No. 1), AGA-GAG-CAA-CAT-GCA (Seq. Id. No. 2), ACA-GCA-GAA-GCC-GTG (Seq. Id. No. 3), CAT-AAA-TGG-CTA-CCA-GA (Seq. Id. No. 4), CAT-AAA-TGG-CTA-CCC-AG (Seq. Id. No. 5), ACT-TGG-AGT-CGA-TAG (Seq. Id. No. 6), CCA-AGG-CTT-ATA-CTC-GC (Seq. Id. No. 7), CCC-CTG-AAT-CGG-GAT (Seq. Id. No. 8), GAC-GCC-AAA-GAC-GCC (Seq. Id. No. 9), ATC-GTC-AGA-GGC-TAT-AA (Seq. Id. No. 10), TAG-CCA-GAA-GAA-AGG (Seq. Id. No. 11), CAT-AAA-TGG-CTA-GCC-AG (Seq. Id. No. 12), CTC-CGA-TGT-GAC-TGC-G (Seq. Id. No. 13), TCC-CAG-ACT-GCT-CGG (Seq. Id. No. 14), TCC-AAG-AGG-TCG-AGA (Seq. Id. No. 15), GCC-AAG-CCA-CAA-GGA (Seq. Id. No. 16), GCC-GCC-AAG-CCA-CA (Seq. Id. No. 17), GGA-CTT-GGG-GTT-AG (Seq. Id. No. 18), CCG-GGT-GCA-TTC-CA (Seq. Id. No. 19), ATG-TAG-AAC-GGA-ACT-A (Seq. Id. No. 20), GAT-TCT-CGG-CCC-CAT-G (Seq. Id. No. 21), CTG-GTT-CGC-CAA-AAA-G (Seq. Id. No. 22) and AGT-ACG-CAT-CAG-AAA (Seq. Id. No. 23).

Claims31

Dependent claims

1-11. (canceled)

A PNA probe set suitable for detecting, identifying or quantitating one or more species of Candida yeast in a sample, wherein at least one PNA probe of the set comprises a probing nucleobase sequence wherein at least a portion is at least ninety percent homologous to the nucleobase sequences, or their complements, selected from the group consisting of:

(Seq. Id. No. 1)
AGA-GAG-CAG-CAT-GCA,
(Seq. Id. No. 2)
AGA-GAG-CAA-CAT-GCA,
(Seq. Id. No. 3)
ACA-GCA-GAA-GCC-GTG,
(Seq. Id. No. 4)
CAT-AAA-TGG-CTA-CCA-GA,
(Seq. Id. No. 5)
CAT-AAA-TGG-CTA-CCC-AG,
(Seq. Id. No. 6)
ACT-TGG-AGT-CGA-TAG,
(Seq. Id. No. 7)
CCA-AGG-CTT-ATA-CTC-GC,
(Seq. Id. No. 8)
CCC-CTG-AAT-CGG-GAT,
(Seq. Id. No. 9)
GAC-GCC-AAA-GAC-GCC,
(Seq. Id. No. 10)
ATC-GTC-AGA-GGC-TAT-AA,
(Seq. Id. No. 11)
TAG-CCA-GAA-GAA-AGG,
(Seq. Id. No. 12)
CAT-AAA-TGG-CTA-GCC-AG,
(Seq. Id. No. 13)
CTC-CGA-TGT-GAC-TGC-G,
(Seq. Id. No. 14)
TCC-CAG-ACT-GCT-CGG,
(Seq. Id. No. 15)
TCC-AAG-AGG-TCG-AGA,
(Seq. Id. No. 16)
GCC-AAG-CCA-CAA-GGA,
(Seq. Id. No. 17)
GCC-GCC-AAG-CCA-CA,
(Seq. Id. No. 18)
GGA-CTT-GGG-GTT-AG,
(Seq. Id. No. 19)
CCG-GGT-GCA-TTC-CA,
(Seq. Id. No. 20)
ATG-TAG-AAC-GGA-ACT-A,
(Seq. Id. No. 21)
GAT-TCT-CGG-CCC-CAT-G,
(Seq. Id. No. 22)
CTG-GTT-CGC-CAA-AAA-G
and
(Seq. Id. No. 23)
AGT-ACG-CAT-CAG-AAA.

44 more elements

A method for detecting, identifying and/or quantitating one or more species of Candida yeast in a sample;

said method comprising the steps of:
a) contacting the sample with one or more PNA probes, wherein the one or more PNA probes have a probing nucleobase sequence that is at least ninety percent homologous to the nucleobase sequences, or their complements, selected from the group consisting of:
(Seq. Id. No. 1)
AGA-GAG-CAG-CAT-GCA,
(Seq. Id. No. 2)
AGA-GAG-CAA-CAT-GCA,
(Seq. Id. No. 3)
ACA-GCA-GAA-GCC-GTG,
(Seq. Id. No. 4)
CAT-AAA-TGG-CTA-CCA-GA,
(Seq. Id. No. 5)
CAT-AAA-TGG-CTA-CCC-AG,
(Seq. Id. No. 6)
ACT-TGG-AGT-CGA-TAG,
(Seq. Id. No. 7)
CCA-AGG-CTT-ATA-CTC-GC,
(Seq. Id. No. 8)
CCC-CTG-AAT-CGG-GAT,
(Seq. Id. No. 9)
GAC-GCC-AAA-GAC-GCC,
(Seq. Id. No. 10)
ATC-GTC-AGA-GGC-TAT-AA,
(Seq. Id. No. 11)
TAG-CCA-GAA-GAA-AGG,
(Seq. Id. No. 12)
CAT-AAA-TGG-CTA-GCC-AG,
(Seq. Id. No. 13)
CTC-CGA-TGT-GAC-TGC-G,
(Seq. Id. No. 14)
TCC-CAG-ACT-GCT-CGG,
(Seq. Id. No. 15)
TCC-AAG-AGG-TCG-AGA,
(Seq. Id. No. 16)
GCC-AAG-CCA-CAA-GGA,
(Seq. Id. No. 17)
GCC-GCC-AAG-CCA-CA,
(Seq. Id. No. 18)
GGA-CTT-GGG-GTT-AG,
(Seq. Id. No. 19)
CCG-GGT-GCA-TTC-CA,
(Seq. Id. No. 20)
ATG-TAG-AAC-GGA-ACT-A,
(Seq. Id. No. 21)
GAT-TCT-CGG-CCC-CAT-G,
(Seq. Id. No. 22)
CTG-GTT-CGC-CAA-AAA-G
and
(Seq. Id. No. 23)
AGT-ACG-CAT-CAG-AAA;
and
b) detecting, identify and/or quantitating hybridization of the probing nucleobase sequence of a PNA probe to the target sequence of an organism of interest in the sample, under suitable hybridization conditions or suitable in-situ hybridization conditions, and correlating the result with the presence, absence or quantity of Candida yeast in the sample.

48 more elements

Dependencies are inferred from the claim text.

PNA Probes, Probe Sets, Methods and Kits Pertaining to the Detection of Candida

Life of this patent

PNA Probes, Probe Sets, Methods and Kits Pertaining to the Detection of Candida

Life of this patent

Abstract

Claims31