Method for Detecting and Identifying Enterohemorrhagic Escherichia coli
AssigneesAgence Nationale de Securite Sanitaire de I'Alimentation, de I'Environnement et du Travail, Bundesinstiful Fur Risikobewertung
InventorsPatrick Fach, Sabine Delannoy, Lothar Beutin
Life of this patent
Estimating the term…
Abstract
The invention relates to methods for predicting whether a sample contains enterohemorrhagic Escherichia coli (EHEC) of at least one of EHEC O157:[H7], O145:[H28], O103:[H2], O111:[H8], O121:[H19], O26: [H11], O45:[H2] or O104: [H4] serotypes, and for identifying said serotypes, through detection of gene espK in association with at least one of the genetic markers Z1151, Z1153, Z1154, Z1155, Z1156, Z6065, Z2098, ureD or espV and/or through detection of serotype-specific CRISPR sequences.
Claims13
A method for identifying the serotype(s) of enterohemorrhagic Escherichia coli (EHEC) suspected to be present in a sample, wherein said method comprises detecting the presence or the absence, in said sample or DNA isolated therefrom, of the following E. coli Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) sequences:
- a) CRISPR sequences for identifying EHEC O157:[H7] wherein said CRISPR sequences are selected among
- the CRISPR sequences SEQ ID NO:
- 1, SEQ ID NO:
- 2, and SEQ ID NO:
- 3, wherein the presence of one or more of said CRISPR SEQ ID NO:
- 1-3 is indicative of the presence of EHEC O157:[H7];
- and/or
- the CRISPR sequence SEQ ID NO:
- 4, wherein the presence of said CRISPR sequence is indicative of the presence of EHEC O157:[H7];
- b) a CRISPR sequence for identifying EHEC O145:[H28], wherein said CRISPR sequence is the sequence SEQ ID NO:
- 5, and wherein the presence of said CRISPR sequence is indicative of the presence of EHEC O145:[H28];
- and
- c) a CRISPR sequence for identifying EHEC O111:[H8], wherein said CRISPR sequence is the sequence SEQ ID NO:
- 6, and wherein the presence of said CRISPR sequence is indicative of the presence of EHEC O111:[H8];
- and
- d) a CRISPR sequence for identifying EHEC O121:[H19], wherein said CRISPR sequence is the sequence SEQ ID NO:
- 7, and wherein the presence of said CRISPR sequence is indicative of the presence of EHEC O121:[H19];
- and
- e) a CRISPR sequence for identifying EHEC O103:[H2] and/or EHEC O45:[H2], wherein said CRISPR sequence is the sequence SEQ ID NO:
- 8, and wherein the presence of said CRISPR sequence is indicative of the presence of EHEC O103:[H2] and/or of EHEC O45:[H2];
- and
- f) a CRISPR sequence for identifying EHEC O104:[H4], wherein said CRISPR sequence is the sequence SEQ ID NO:
- 9, and wherein the presence of said CRISPR sequence is indicative of the presence of EHEC O104:[H4];
- and
- g) a CRISPR sequence for identifying EHEC O26:[H11], wherein said CRISPR sequence is the sequence SEQ ID NO:
- 10, and wherein the presence of said CRISPR sequence is indicative of the presence of EHEC O26:[H11].
A method for predicting whether a sample contains EHEC of at least one of EHEC O157:[H7], O145:[H28], O103:[H2], O111:[H8], O121:[H19], O26:[H11], O45:[H2] and O104:[H4] serotypes, wherein said method comprises the detection of the espK gene and of one or more of the following target genes:
- espV, ureD, Z2098, Z1151, Z1153, Z1154, Z1155, Z1156, and Z6065.
Dependencies are inferred from the claim text.