Production of fatty alcohols from engineered microorganisms
AssigneeCodexis, Inc.
InventorsJonathan Vroom, Svetlana Balatskaya, Yoram Barak, Louis Clark, Trish Choudhary, Kristian Karlshoej, Fernando Valle, Kaman Chan
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Abstract
Recombinant microorganisms are provided which have been engineered to produce fatty alcohols. Also provided are recombinant microorganisms which comprise a heterologous polynucleotide encoding a fatty alcohol reductase enzyme and an introduced polynucleotide encoding a β-ketoacyl acyl carrier protein synthase.
Claims49
An engineered prokaryotic cell comprising two recombinant polynucleotides the first polynucleotide encoding a heterologous fatty alcohol forming reductase (“FAR”) and the second polynucleotide encoding a β-ketoacyl acyl carrier protein synthase III (“FabH”).
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A vector comprising a first polynucleotide sequence encoding a fatty alcohol forming reductase (“FAR”) and a second polynucleotide sequence encoding a β-ketoacyl acyl carrier protein synthase III (KAS-III), wherein the polynucleotide sequences are operably linked to a promoter that is functional in a prokaryotic host cell.
A recombinant bacterial culture that produces a composition of fatty alcohols comprising carbon chain lengths having at least 60% of C12, C14 and C16 fatty alcohols, wherein the bacterial culture comprises an engineered bacterial microorganism comprising a gene encoding a heterologous fatty alcohol forming reductase (FAR) comprising at least 90% sequence identity to SEQ ID NO:
- 2, SEQ ID NO:4, SEQ ID NO:
- 6 or SEQ ID NO:8 and a gene encoding a heterologous FabH comprising at least 90% sequence identity to SEQ ID NO:
- 10.
A method of shifting the C12, C14, and C16 carbon chain length profile of a fatty alcohol composition produced by an engineered bacterial cell comprising a polynucleotide encoding a heterologous fatty alcohol forming reductase (“FAR”) having at least 90% sequence identity to SEQ ID NO:
- 2, SEQ ID NO:
- 4, SEQ ID NO 6 or SEQ ID NO:
- 8 said method comprising introducing a polynucleotide encoding a β-ketoacyl acyl carrier protein synthase III (“FabH”) having at least 90% sequence identity to SEQ ID NO:
- 10 into the engineered cell, wherein the composition of C12 fatty alcohols produced by the engineered bacterial cell has been increased by 25% and the composition of C16 fatty alcohols has been decreased by at least 10% as compared to a corresponding engineered bacterial cell which includes the corresponding polynucleotide encoding the FAR having at least 90% sequence identity to SEQ ID NO:
- 2, SEQ ID NO:
- 4, SEQ ID NO 6 or SEQ ID NO:
- 8 but not the corresponding introduced polynucleotide encoding a FabH having at least 90% sequence identity to SEQ ID NO:
- 10.
Dependencies are inferred from the claim text.