Xylose fermenting yeast constructed using a modified genome shuffling method
AssigneeNgee Ann Polytechnic
InventorsAnli Geng, Wei Zhang
Life of this patent
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Abstract
Disclosed is a method of providing a recombinant microorganism. The method comprises the steps of: (a) providing a hybrid microorganism comprising DNA from a host microorganism and a donor microorganism; and (b) fusing DNA extracted from a second microorganism into the hybrid microorganism to form the recombinant microorganism. Also disclosed are recombinant yeasts produced by the method of the present disclosure and a method of fermenting sugar using the recombinant yeast produced by the method of the present disclosure.
Claims35
A method of providing a recombinant microorganism, said method comprising:
- (a) providing a hybrid microorganism comprising whole genomic DNA from a host microorganism and a donor microorganism, wherein the hybrid microorganism is thus a microorganism having genomes from two different parent microorganism, the hybrid microorganism is produced by transferring the whole genome of the donor microorganism to the host microorganism by whole genome transformation;
- and the hybrid microorganism exhibits an enhanced activity as compared to its host microorganism and/or donor microorganism individually;
- and
- (b) fusing whole genomic DNA extracted from a second microorganism into the hybrid microorganism to form said recombinant microorganism.
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A recombinant yeast strain produced according to a method of providing a recombinant microorganism, said method comprising:
- (a) providing a hybrid microorganism comprising whole genomic DNA from a host microorganism and a donor microorganism, wherein the hybrid microorganism is thus a microorganism having genomes from two different parent microorganism, the hybrid microorganism is produced by transferring the whole genome of the donor microorganism to the host microorganism by whole genome transformation;
- and the hybrid microorganism exhibits an enhanced activity as compared to its host microorganism and/or donor microorganism individually;
- and
- (b) fusing whole genomic DNA extracted from a second microorganism into the hybrid microorganism to form said recombinant microorganism.
A method of fermenting sugar with a recombinant yeast strain to produce ethanol, wherein the recombinant yeast is produced according to a method of providing a recombinant microorganism, said method comprising:
- (a) providing a hybrid microorganism comprising whole genomic DNA from a host microorganism and a donor microorganism, wherein the hybrid microorganism is thus a microorganism having genomes from two different parent microorganism, the hybrid microorganism is produced by transferring the whole genome of the donor microorganism to the host microorganism by whole genome transformation;
- and the hybrid microorganism exhibits an enhanced activity as compared to its host microorganism and/or donor microorganism individually;
- and
- (b) fusing whole genomic DNA extracted from a second microorganism into the hybrid microorganism to form said recombinant microorganism.
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A method of fermenting a sugar mixture having a high sugar content with a recombinant yeast strain to produce ethanol, wherein the total sugar content is greater than 50 grams per liter, wherein the recombinant yeast strain is produced according to a method of providing a recombinant microorganism, said method comprising:
- (a) providing a hybrid microorganism comprising whole genomic DNA from a host microorganism and a donor microorganism, wherein the hybrid microorganism is thus a microorganism having genomes from two different parent microorganism, the hybrid microorganism is produced by transferring the whole genome of the donor microorganism to the host microorganism by whole genome transformation;
- and the hybrid microorganism exhibits an enhanced activity as compared to its host microorganism and/or donor microorganism individually;
- and
- (b) fusing whole genomic DNA extracted from a second microorganism into the hybrid microorganism to form said recombinant microorganism.
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