Sugar transport sequences, yeast strains having improved sugar uptake, and methods of use
AssigneesWisconsin Alumni Research Foundation, The United States of America as represented by the Secretary of Agriculture
InventorsThomas William Jeffries, JuYun Bae, Bernice Chin-Yun Lin, Jennifer Rebecca Headman Van Vleet
Life of this patent
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Abstract
Disclosed are nucleic acid constructs comprising coding sequences operably linked to a promoter not natively associated with the coding sequence. The coding sequences encode Pichia stipitis proteins that allow recombinant strains of Saccharomyces cerevisiae expressing the protein to grow on xylose, and allow or increase uptake of xylose by Pichia stipitis or Saccharomyces cerevisiae expressing the coding sequences. Expression of the coding sequences enhances uptake of xylose and/or glucose, allowing increased ethanol or xylitol production.
Claims15
1-20. (canceled)
A nucleic acid construct comprising a first coding sequence operably linked to a first promoter not natively associated with the coding sequence, the coding sequence encoding a glucose/xylose transporter polypeptide having at least 95% amino acid identity to SEQ ID NO:2.
Dependencies are inferred from the claim text.