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AssigneeTechnische UniversitäT Dresden
InventorsFrank Buchholz, Madina Karimova
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The invention relates to the use of a protein with recombinase activity to catalyze a site-specific DNA recombination and a method for producing a site-specific DNA recombination. The invention is applicable alone or in combination with other recombinase systems for genetic manipulation, for example in medical research. The objective of the invention is solved by the use of a protein with recombinase activity to catalyze a site-specific DNA recombination at, preferably at two, recognition sites that are identical or reverse complementary to each other. The invention also includes a method for producing a site-specific DNA recombination comprising the steps of a) providing a cell comprising at least two recognition sites that are identical or reverse complementary to each other; and b) contacting a protein with recombinase activity with the recognition sites, thereby producing the site-specific DNA-recombination.
A method for producing a site-specific DNA-recombination, comprising:
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Use of a protein with recombinase activity, wherein the protein comprises an amino acid sequence exhibiting at least 70%, preferably at least 80%, preferably at least 90%, particularly preferred at least 95%, even more preferred at least 99% amino acid sequence identity to SEQ ID No. 1 to catalyze a site-specific DNA-recombination at recognition sites that are identical or reverse complementary to each other, wherein at least one recognition site comprises a nucleic acid sequence according to or reverse complementary to SEQ ID No. 2.
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A nucleic acid exhibiting a length not more than 40 base pairs, the nucleic acid comprising a nucleic acid sequence according to SEQ ID No. 2 or a nucleic acid sequence reverse complementary thereto.
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Dependencies are inferred from the claim text.