Heat-Stable Persephonella Carbonic Anhydrases and Their Use

A method for extracting carbon dioxide comprising treating a carbon dioxide-containing medium with a carbonic anhydrase, wherein the carbonic anhydrase is selected from the group consisting of:

• a) a polypeptide derived from Persephonella marina DSM 14350;
• b) a polypeptide having an amino acid sequence corresponding to amino acid residues 20 to 243 of SEQ ID NO:
• 2 or amino acid residues 29 to 251 of SEQ ID NO:
• 4;
• c) a polypeptide which is at least 60% identical to amino acid residues 20 to 243 of SEQ ID NO:
• 2 or amino acid residues 29 to 251 of SEQ ID NO:
• 4;
• d) a fragment of (a), (b) or (c) having carbonic anhydrase activity;
• e) a polypeptide encoded by a nucleic acid sequence which hybridizes under medium stringency conditions with:
• i) a polynucleotide sequence encoding a mature polypeptide of SEQ ID NO:
• 2 or SEQ ID NO:
• 4;
• or
• ii) a polynucleotide sequence of SEQ ID NO:
• 1 or SEQ ID NO:
• 3;
• or
• iii) a subsequence of (i) or (ii), of at least 100 contiguous nucleotides, or
• iv) a complementary strand of (i) or (ii);
• f) a polypeptide encoded by a nucleic acid sequence which, because of the degeneracy of the genetic code, does not hybridize with the polynucleotide sequence of SEQ ID NO:
• 1 or SEQ ID NO:
• 3, but which codes for a polypeptide having an amino acid sequence according to b) or c);
• and
• g) a polypeptide encoded by a nucleic acid sequence which is at least 60% identical to SEQ ID NO:
• 1 or SEQ ID NO:
• 3.