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US 2015/0175971 A1Published application
Multipotent cells having mesenchymal and endothelial lineage potential
AssigneeWisconsin Alumni Research Foundation
InventorsMaksym A. Vodyanyk, Igor I. Slukvin
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Abstract
Methods for obtaining multipotent Apelin receptor-positive lateral plate mesoderm cells, mesenchymal stem cells, and mesangioblasts under serum-free conditions are disclosed.
Claims14
-29. (canceled)
A method of generating primate cells expressing Apelin receptor, the method comprising the steps of:
- culturing primate pluripotent stem cells in co-culture with OP9 bone marrow stromal cells until Apelin receptor is expressed and detectable in the co-cultured cells;
- and
- sorting the co-cultured cells expressing Apelin receptor from cells of the co-culture not expressing Apelin receptor.
A method of generating mesangioblasts, the method comprising the steps of:
- culturing primate pluripotent stem cells in co-culture with OP9 bone marrow stromal cells until Apelin receptor is expressed and detectable in the co-cultured cells;
- sorting the co-cultured cells expressing Apelin receptor from cells of the co-culture not expressing Apelin receptor;
- and
- culturing the Apelin receptor+ cells in a semi-solid culture medium comprising bFGF until a subset differentiate to form mesangioblasts.
1 more element
Dependencies are inferred from the claim text.