Process for Preparation of a Grifola frondosa Polysaccharide F2 and Its Hypoglycemic Activity

Abstract

A Grifola frondosa polysaccharide F2 with hypoglycemic activity, process for preparation and use thereof. The process for preparation of Grifola frondosa polysaccharide F2 is as follows: The fruit bodies of Grifola frondosa were homogenized to a fine powder and extracted with hot water. The mixture was filtered and precipitated with absolute ethanol. The precipitation was obtained. The said precipitation was applied on DEAE Sepharose Fast chromatographic column, equilibrated with Tris-HCl(10 mM, pH=8.0), collecting the efficient eluting peak to obtain the fraction F1; eluted with Tris-HCl(10 mM, pH=8.0) which contains 0.1M NaCl, fraction F2 was obtained; then concentrated under reduced pressure, dialyzed and lyophilized, Grifola frondosa polysaccharide F2 was obtained. This isolates a new Grifola frondosa polysaccharide F2 with hypoglycemic activity from the fruit bodies of Grifola frondosa. The Grifola frondosa polysaccharide F2 can be used in manufacturing a drug for treating diabetes. The polysaccharide makes a foundation for developing new anti-diabetes agents.

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