Recombinant antibody compositions and methods of use thereof
AssigneeLife Technologies Corporation
InventorsKaroline Schjetne, Tanja Aarvak, Lars Norderhaug
Life of this patent
Estimating the term…
Abstract
Provided are methods and composition useful in isolating or otherwise preparing a population of target cells. In part, it relates to recombinant antibodies comprising an exogenous proteolytic cleavage site and nucleic acid molecules encoding the antibodies.
Claims18
A method for detaching a target cell attached to a recombinant antibody-coated solid surface, comprising[:
- ] contacting the target cell attached to the surface with a protease, or portion of a protease, specific for an exogenous proteolytic cleavage site in the antibody, thereby detaching the target cell from the surface.
2-37. (canceled)
A method for isolating a target cell from a mixed cell population, comprising:
- (a) contacting a mixed cell population with an antibody attached to a solid surface, wherein the antibody specifically binds to the target cell in the population and wherein the antibody comprises an exogenous proteolytic cleavage site;
- (b) separating the target cells bound to the surface from cells not bound to the surface;
- and
- (c) contacting the target cells bound to the surface with a protease, or portion of a protease, specific for the cleavage site to detach the target cells from the surface.
A recombinant antibody comprising an exogenous proteolytic cleavage site in the hinge region.
A composition comprising a cell, a recombinant antibody, and a solid surface, wherein the recombinant antibody is specifically bound to the cell and is also attached to the solid surface, and wherein the recombinant antibody comprises an exogenous proteolytic cleavage site.
Dependencies are inferred from the claim text.