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US 2015/0174575 A1Published application
Thermal Reaction Device and Method for Using the Same
AssigneeFluidigm Corporation
InventorsMarc A. Unger, Lincoln McBride, Geoffrey Facer
Life of this patent
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Abstract
Devices and methods for performing the relative concentration of a target in a sample, the sample containing both target and non-target components, the method performed by partitioning the sample into a large number of reaction volumes such that the target is concentrated relative to the non-target, and performing a detection assay upon each reaction volume to detect the target.
Claims2
A method for detecting the presence and/or quantity of a target polynucleotide in a sample, the method comprising:
- (a) providing a microfluidic device having at least one sample channel and a plurality of valves distributed along the length of the sample channel, wherein the sample channel is adapted to be partitioned into a plurality of separate reaction chambers by the closing of one or more valves,
- (b) introducing a sample having at least one amplifiable target into the sample channel,
- (c) closing the plurality of valves to form separate reaction chambers so that the amplifiable target is isolated into at least one chamber,
- (d) conducting a polynucleotide amplification reaction,
- (e) detecting the presence of a polynucleotide amplification product in at least one reaction chamber to determine the presence and/or quantity of the target polynucleotide in the sample.
2 more elements
2-28. (canceled)
Dependencies are inferred from the claim text.