Transfection systems, methods and media
OwnerNeil Emans, Asongwe Lionel Ateh Tantohinventors
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Abstract
Systems, methods and media for transfection are provided. In an example embodiment, a method of printing layered arrays of spots onto a substrate includes printing a first array of spots onto the substrate and allowing the first array of spots to dry. The method includes printing, over the first array of spots, a second array of spots, with the spots of the second array being at least partially coincident with the spots of the first array, and allowing the second array of spots to dry. The method may include printing, over the second array of spots, a third array of spots, the spots of the third array being at least partially coincident with the spots of the second array, and allowing the third array of spots to dry.
Claims31
A method of printing, onto a substrate, layered arrays of spots, which method includes:
- printing a first array of spots onto the substrate;
- allowing the first array of spots to dry;
- printing, over the first array of spots, a second array of spots, the spots of the second array being at least partially coincident with the spots of the first array;
- allowing the second array of spots to dry;
- printing, over the second array of spots, a third array of spots, the spots of the third array being at least partially coincident with the spots of the second array;
- and
- allowing the third array of spots to dry.
A printing apparatus for printing, onto a substrate, an array of spots of reagent composition, which apparatus includes:
- an array of capillary tubes arranged alongside one another and each having at least one open end, with the open ends of the tubes being aligned;
- displacement means for displacing the array of capillary tubes from an inoperative position to an operative position and back to the inoperative position;
- and
- substrate holding means for holding the substrate so that, in use, when the array of capillary tubes is displaced into its operative position, the open ends of the capillary tubes can simultaneously impinge against a substrate held by the substrate holding means with at least some reagent composition from the capillary tubes being deposited on the substrate as spots, thereby to form an array of spots of the reagent compositions on the substrate.
A machine readable medium including instructions that, when implemented, cause the machine to perform operations comprising:
- printing a first array of spots onto a substrate;
- receiving the first array of spots when dry;
- printing, over the first array of dried spots, a second array of spots, the spots of the second array being at least partially coincident with the spots of the first array;
- receiving the second array of spots when dry;
- and
- printing, over the second array of dried spots, a third array of spots, the spots of the third array being at least partially coincident with the spots of the second array.
A system including at least one module, executing on at least one computer processor, to:
- print a first array of spots onto a substrate;
- print, over the first array of dried spots, a second array of spots, the spots of the second array being at least partially coincident with the spots of the first array;
- and
- print, over the second array of dried spots, a third array of spots, the spots of the third array being at least partially coincident with the spots of the second array.
Dependencies are inferred from the claim text.