Modification of surfaces for simultaneous repellency and targeted binding of desired moieties

A diagnostic device comprising

• a substrate;
• anchoring molecules comprising a head group attached to the substrate and a tail group directly or indirectly attached to the head group, wherein the tail group has an affinity with a lubricating liquid;
• a lubricating layer immobilized over the substrate surface comprising said lubricating liquid having an affinity with the tail group of said anchoring molecules, wherein the anchoring molecules and the lubricating liquid are held together by non-covalent attractive forces,
• areas of said substrate comprising a binding group directly or indirectly secured to the substrate and extending over the surface of the lubricating layer or retained within the lubricating layer having an affinity with a target moiety;
• wherein the anchoring molecules and the lubricating layer form a slippery surface configured and arranged for contact with a material that is immiscible with the lubricating liquid;
• wherein said diagnostic tool provides a high signal-to-noise ratio for capture of the target moieties over other non-target moieties.

A device comprising:

• (i) a chamber with an inlet and an outlet;
• and
• (ii) at least one fiber disposed in the chamber between the inlet and the outlet, wherein at least a portion of the fiber comprises a liquid-repellant surface, wherein the liquid-repellant surface is coated with perfluorocarbon-containing silanes;
• and
• wherein at least a portion of the fiber further comprises on its surface microbe-binding molecules conjugated thereto, the microbe-binding molecules each comprising:
• (a) at least one microbe surface-binding domain;
• and (b) at least a portion of a Fc region of an immunoglobulin.

An article comprising:

• a substrate;
• anchoring molecules comprising a head group attached to the substrate and a tail group directly or indirectly attached to the head group, wherein the tail group has an affinity with a lubricating liquid;
• a lubricating layer immobilized over the substrate surface comprising said lubricating liquid having an affinity with the tail group of said anchoring molecules, wherein the anchoring molecules and the lubricating liquid are held together by non-covalent attractive forces,
• microbe-binding molecules directly or indirectly secured to the substrate and extending over the surface of the lubricating layer or retained within the lubricating layer, wherein the microbe-binding molecules each comprising:
• (a) at least the carbohydrate recognition domain and neck region of mannose-binding lectin;
• and (b) at least a portion of a Fc region of an immunoglobulin;
• wherein the anchoring molecules and the lubricating layer form a slippery surface configured and arranged for contact with a material that is immiscible with the lubricating liquid.