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US 2015/0168393 A1Published application
Spatial Positioning of Spectrally Labeled Beads
AssigneeLife Technologies Corporation
InventorsStephen Empedocles, Andrew Watson, Jian Jin
Life of this patent
Estimating the term…
Abstract
Devices, systems, kits, and methods for detecting and/or identifying a plurality of spectrally labeled bodies well-suited for performing multiplexed assays. By spectrally labeling the beads with materials which generate identifiable spectra, a plurality of beads may be identified within the fluid. Reading of the beads is facilitated by restraining the beads in arrays, and/or using a focused laser.
Claims21
1-16. (canceled)
A multiplex assay detection apparatus, comprising:
- an excitation energy source configured to excite a plurality of spectrally labeled bodies bound to a surface of an assay plate, wherein the spectrally labeled bodies each include a plurality of markers, wherein, upon excitation, each marker generates an identifiable signal, wherein a combination of signals generate a spectrum;
- a sensor configured to detect the spectrum of each of the plurality of spectrally labeled bodies;
- and
- a processor configured to utilize machine-readable code including a listing of elements included in a library of spectral codes that, together, with the spectrum sensed by the sensor identifies the spectrally labeled bodies.
1 more element
A multiplex assay, comprising:
- an assay substrate;
- and
- a plurality of spectrally labeled bodies affixed to the assay substrate in a spatially resolved configuration, wherein each of the spectrally labeled bodies includes a plurality of markers used to encode discrete and different emission spectra, wherein each emission spectrum has a plurality of signals at differing wavelengths.
A multiplex assay apparatus, comprising:
- a sample container configured to hold a sample fluid containing a plurality of spectrally labeled bodies;
- and
- a fluidics assembly configured to wash away spectrally labeled bodies not bound to an identifiable substance, transfer the bound spectrally labeled bodies to a reading container and affix the bound spectrally labeled bodies to the reading container in a spatially resolved configuration, wherein each of the bound spectrally labeled bodies includes a plurality of markers used to encode discrete and different emission spectra, wherein each emission spectrum has a plurality of signals at differing wavelengths.
Dependencies are inferred from the claim text.