# US 2015/0147809 A1 · Cell seeding device and method > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2015/0147809 A1 - Document ID: US20150147809A1 - Status: Published application - Kind: A1 - Application number: 14/088,999 - Filed: 2013-11-25 - Published: 2015-05-28 - Earliest claimed: 2013-11-25 - Assignees: Vericel Corporation - Inventors: Yongzhong WANG; Christopher KELLY; Stephen RAPKO; Frank QUINNO; Barbara SEYMOUR - Web page: https://patentgrep.com/patent/US20150147809A1 ## CPC - C12M 25/04: {in combination with well or multiwell plates, i.e. culture inserts} - C12M 23/20: {Material Coatings (immunocoatings C12M25/00)} - C12M 23/46: {Means for fastening} - C12M 25/14: {Scaffolds; Matrices (in general C12N5/0068)} - C12M 41/48: {Automatic or computerized control (automatic analysis G01N35/00)} - C12M 23/12: {Well or multiwell plates (C12M25/04 takes precedence)} - C12M 37/00: {Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination (C12M23/38 takes precedence; filtration in general and filters per se B01D24/00-B01D41/00; autoclaves B01J3/04; treatment of microorganisms with electrical or wave energy C12N13/00)} - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} ## Abstract Described is a device comprising an anchor, a tray including a well adapted to receive the anchor, and a cover adapted to engage the tray and cover the well. The device may be used to form a membrane-cell matrix having a substantially uniform distribution of the cells on the membrane in at least two dimensions. ## Claims ### Claim 1 A device comprising: an anchor; a tray including a well adapted to receive the anchor; and a cover adapted to engage the tray and cover the well. ### Claim 2 (depends on 1) The device according to claim 1, wherein the tray includes a top surface surrounding an opening of the well. ### Claim 3 (depends on 2) The device according to claim 2, wherein the tray includes a flange disposed at least partially around a periphery of the top surface. ### Claim 4 (depends on 3) The device according to claim 3, wherein the flange includes at least one first thread. ### Claim 5 (depends on 4) The device according to claim 4, wherein the top surface includes a first rim formed at least partially around the periphery of the top surface. ### Claim 6 (depends on 1) The device according to claim 1, wherein the well includes at least one first sidewall and a base. ### Claim 7 (depends on 6) The device according to claim 6, wherein the at least one first sidewall is disposed approximately perpendicular to the base. ### Claim 8 (depends on 6) The device according to claim 6, wherein the base includes a second rim formed at least partially around a periphery of an external surface. ### Claim 9 (depends on 6) The device according to claim 6, wherein the anchor includes at least one second sidewall enclosing a space. ### Claim 10 (depends on 9) The device according to claim 9, wherein a first cross-sectional area of the base is approximately equal to a second cross-sectional area of the space. ### Claim 11 (depends on 9) The device according to claim 9, wherein the anchor includes at least one tab disposed on the at least one sidewall. ### Claim 12 (depends on 11) The device according to claim 11, wherein the at least one tab is disposed in a same plane as the at least one sidewall. ### Claim 13 (depends on 1) The device according to claim 1, wherein the well is at least partially transparent and the anchor is at least partially colored. ### Claim 14 (depends on 5) The device according to claim 5, wherein the cover includes a cavity adapted to receive the flange. ### Claim 15 (depends on 14) The device according to claim 14, wherein the cavity includes at least one second thread adapted to engage the at least one first thread. ### Claim 16 (depends on 14) The device according to claim 14, wherein the cavity includes a slot adapted to receive the first rim. ### Claim 17 (depends on 11) The device according to claim 11, wherein the cover includes at least one rib formed on an outer surface. ### Claim 18 A device comprising: an anchor including at least one first sidewall enclosing a space having X, Y, and Z dimensions; a tray including a well adapted to receive the anchor, the well having at least one second sidewall and a base having X and Y dimensions; and a cover adapted to engage the tray, wherein the X and Y dimensions of the space are approximately equal to the X and Y dimensions of the base such that when the anchor is in the well, at least one of the at least one first sidewall and at least one of the at least one second sidewall are in direct contact. ### Claim 19 A method of generating a membrane-cell matrix having a substantially uniform distribution of the cells on the membrane in at least two dimensions comprising: providing a device comprising: an anchor; and a tray including a well adapted to receive the anchor; placing a membrane in the well; placing the anchor in the well at least partially in contact with the membrane; and filling the well at least partially with a medium containing cells. ### Claim 20 (depends on 20) The method according to claim 20, further comprising: removing the anchor from the well; and removing the membrane-cell matrix.