# US 2015/0147769 A1 · Method of testing sample and microfluidic device

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- Number: US 2015/0147769 A1
- Document ID: US20150147769A1
- Status: Published application
- Kind: A1
- Application number: 14/528,157
- Filed: 2014-10-30
- Published: 2015-05-28
- Earliest claimed: 2013-11-22
- Assignees: Samsung Electronics Co., Ltd.
- Applicants: Samsung Electronics Co., Ltd.
- Inventors: Kui Hyun KIM; Taguchi TAKAYUKI; Sung Joon PARK; Chang Soo PARK; Sang Hyun BAEK; Tae Han LEE
- Web page: https://patentgrep.com/patent/US20150147769A1

## CPC

- C12Q 1/40: involving amylase
- G01N 21/78: producing a change of colour
- Y10T 436/19: Halogen containing
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

## Abstract

A method of testing a sample to determine a concentration of a target material included in the sample and a microfluidic device in which a reaction of the sample and a reagent occurs are provided. The method includes mixing a sample with a reagent that changes optical characteristics in accordance with a concentration of chlorine ions in the sample, and a capturing material that captures some of the chlorine ions in the sample; measuring the optical characteristics after mixing the sample with the reagent and the capturing material; and determining a concentration of the chlorine ions in the sample based on the measured optical characteristics.

## Claims

### Claim 1

A method of determining the concentration of chlorine ions in a sample, the method comprising:
mixing a sample with a reagent that changes optical characteristics in accordance with a concentration of chlorine ions in the sample, and a capturing material that captures some of the chlorine ions in the sample;
measuring the optical characteristics after mixing the sample with the reagent and the capturing material; and
determining a concentration of the chlorine ions in the sample based on the measured optical characteristics.

### Claim 2 (depends on 1)

The method according to claim 1, wherein the capturing material is a compound comprising an amine (—NH2) group.

### Claim 3 (depends on 2)

The method according to claim 2, wherein the capturing material comprises at least one selected from the group consisting of urea, thio-urea, an N-(2-acetamido)-2-aminoethanesulfonic acid (ACES) buffer, and a 2-[(2-amino-2-oxoethyl)-(carboxymethyl)amino]acetic acid (ADA) buffer.

### Claim 4 (depends on 2)

The method according to claim 2, wherein the amine group of the capturing material binds to the chlorine ions.

### Claim 5 (depends on 2)

The method according to claim 2, wherein the reagent comprises an enzyme activated by the chlorine ions and a substrate degraded by the activated enzyme.

### Claim 6 (depends on 5)

The method according to claim 5, wherein the enzyme is activated by chlorine ions that are not bound to the capturing material.

### Claim 7 (depends on 6)

The method according to claim 6, wherein the enzyme is α-amylase.

### Claim 8 (depends on 7)

The method according to claim 7, wherein the substrate is 2-chloro-4-nitrophenyl-alpha-maltotrioside (CNPG3).

### Claim 9 (depends on 8)

The method according to claim 8, wherein the CNPG3 is hydrolyzed by the α-amylase to generate 2-chloro-4-nitrophenol (CNP) and α-maltotriose (G3).

### Claim 10

A microfluidic device comprising:
at least one chamber containing a reagent that changes optical characteristics according to a concentration of chlorine ions in a sample, and a capturing material that captures some of the chlorine ions in the sample; and
a sample inlet into which the sample is injected.

### Claim 11 (depends on 10)

The device according to claim 10, wherein the capturing material is a compound comprising an amine (—NH2) group.

### Claim 12 (depends on 11)

The device according to claim 11, wherein the capturing material comprises at least one selected from the group consisting of urea, thio-urea, an N-(2-acetamido)-2-aminoethanesulfonic acid (ACES) buffer, and a 2-[(2-amino-2-oxoethyl)-(carboxymethyl)amino]acetic acid (ADA) buffer.

### Claim 13 (depends on 11)

The device according to claim 11, wherein the amine group of the capturing material binds to the chlorine ions.

### Claim 14 (depends on 11)

The device according to claim 11, wherein the reagent comprises an enzyme activated by the chlorine ions and a substrate degraded by the activated enzyme.

### Claim 15 (depends on 14)

The device according to claim 14, wherein the enzyme is activated by chlorine ions that are not bound to the capturing material.

### Claim 16 (depends on 15)

The device according to claim 15, wherein the enzyme, substrate and capturing material are contained in one of the at least one chambers.

### Claim 17 (depends on 16)

The device according to claim 16, further comprising:
a channel connecting the chamber containing the enzyme, substrate and capturing material with the sample inlet.

### Claim 18 (depends on 15)

The device according to claim 15, wherein the enzyme is α-amylase.

### Claim 19 (depends on 18)

The device according to claim 18, wherein the substrate is 2-chloro-4-nitrophenyl-alpha-maltotrioside (CNPG3).